Response of porcine intestinal in vitro organ culture tissues following exposure to Lactobacillus plantarum JC1 and Salmonella enterica serovar Typhimurium SL1344.

Applied and Environmental Microbiology
J W CollinsRoberto M La Ragione

Abstract

The development of novel intervention strategies for the control of zoonoses caused by bacteria such as Salmonella spp. in livestock requires appropriate experimental models to assess their suitability. Here, a novel porcine intestinal in vitro organ culture (IVOC) model utilizing cell crown (CC) technology (CCIVOC) (Scaffdex) was developed. The CCIVOC model was employed to investigate the characteristics of association of S. enterica serovar Typhimurium strain SL1344 with porcine intestinal tissue following exposure to a Lactobacillus plantarum strain. The association of bacteria to host cells was examined by light microscopy and electron microscopy (EM) after appropriate treatments and staining, while changes in the proteome of porcine jejunal tissues were investigated using quantitative label-free proteomics. Exposure of porcine intestinal mucosal tissues to L. plantarum JC1 did not reduce the numbers of S. Typhimurium bacteria associating to the tissues but was associated with significant (P < 0.005) reductions in the percentages of areas of intestinal IVOC tissues giving positive staining results for acidic mucins. Conversely, the quantity of neutrally charged mucins present within the goblet cells of the IVOC tissues incr...Continue Reading

References

Apr 1, 1988·Journal of Comparative Pathology·P J BrownF J Bourne
Mar 15, 1997·Genes & Development·B D Jones
Jun 1, 1997·Gastroenterology·J E BrankaC L Laboisse
Jan 1, 1997·Journal of Clinical Gastroenterology·O Furukawa, S Okabe
Oct 28, 1999·FEMS Immunology and Medical Microbiology·E M TuomolaS J Salminen
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·J E Galan, D Zhou
Dec 12, 2002·The Biochemical Journal·Philippe SoubeyranIvan Dikic
Feb 4, 2003·Experimental Biology and Medicine·William B Pratt, David O Toft
Aug 19, 2003·Current Issues in Molecular Biology·Alessandra Carattoli
Sep 23, 2003·Current Opinion in Infectious Diseases·Christopher M Parry
Sep 26, 2003·Best Practice & Research. Clinical Gastroenterology·Alain L Servin, Marie-Hélène Coconnier
Nov 18, 2003·Molecular Biology of the Cell·Tatsuru Togo, Richard A Steinhardt
Jun 4, 2004·Human Molecular Genetics·H J SpenceS J Winder
Oct 22, 2004·Emerging Infectious Diseases·John D BoyceBen Adler
Apr 15, 2005·The Veterinary Record·S K Kritas, R B Morrison
Apr 22, 2005·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·S Di CaroA Gasbarrini
Jun 28, 2005·Journal of the American Society for Mass Spectrometry·Ji GaoGregory J Opiteck
Jul 21, 2005·Bioinformatics·Lennart MartensKris Gevaert
Jun 27, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·G Lamprecht, U Seidler
Sep 2, 2006·Journal of Proteome Research·Boris ZybailovMichael P Washburn
Sep 16, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·C Caballero-FrancoK Chadee
Nov 4, 2006·Journal of Proteome Research·Bing ZhangNagiza F Samatova
Dec 6, 2006·Immunology and Cell Biology·Bryan CoburnB B Finlay
Mar 22, 2007·Physiological Genomics·Wendy RodenburgJaap Keijer
Jun 5, 2007·Journal of Proteome Research·Marianne DanielsenEmøke Bendixen
Sep 14, 2007·BMC Microbiology·Wendy RodenburgIngeborg M J Bovee-Oudenhoven

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Citations

Sep 23, 2014·PloS One·Matthew R HaywardMartin J Woodward
Oct 22, 2016·In Vitro Cellular & Developmental Biology. Animal·Matheus O CostaJanet E Hill
Jun 8, 2014·Applied and Environmental Microbiology·A A Guerra-OrdazS M Martín-Orúe
Jan 16, 2015·Journal of Proteome Research·Ying-Hua ChangYing Ge

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