Response of Secondary Metabolism of Hypogean Actinobacterial Genera to Chemical and Biological Stimuli

Applied and Environmental Microbiology
Brett C CovingtonBrian O Bachmann

Abstract

Microorganisms within microbial communities respond to environmental challenges by producing biologically active secondary metabolites, yet the majority of these small molecules remain unidentified. We have previously demonstrated that secondary metabolite biosynthesis in actinomycetes can be activated by model environmental chemical and biological stimuli, and metabolites can be identified by comparative metabolomics analyses under different stimulus conditions. Here, we surveyed the secondary metabolite productivity of a group of 20 phylogenetically diverse actinobacteria isolated from hypogean (cave) environments by applying a battery of stimuli consisting of exposure to antibiotics, metals, and mixed microbial culture. Comparative metabolomics was used to reveal secondary metabolite responses from stimuli. These analyses revealed substantial changes in global metabolomic dynamics, with over 30% of metabolomic features increasing more than 10-fold under at least one stimulus condition. Selected features were isolated and identified via nuclear magnetic resonance (NMR), revealing several known secondary metabolite families, including the tetarimycins, aloesaponarins, hypogeamicins, actinomycins, and propeptins. One prioritize...Continue Reading

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Citations

Apr 11, 2019·Frontiers in Microbiology·Pharada Rangseekaew, Wasu Pathom-Aree
Jul 28, 2020·Applied Biochemistry and Biotechnology·Fatemeh MohammadipanahFatemeh Salimi
Apr 14, 2021·Annual Review of Biochemistry·Brett C CovingtonMohammad R Seyedsayamdost
Oct 26, 2021·Natural Product Reports·Marc G Chevrette, Jo Handelsman

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