Response of the adipose tissue transcriptome to dihydrotestosterone in mice

Physiological Genomics
Yonghua ZhangAndré Tchernof

Abstract

Androgens have been postulated to be important modulators of adipose tissue metabolism and fat cell function. In the present study, we investigated the response of male and female mice retroperitoneal adipose tissue to the nonaromatizable androgen dihydrotestosterone (DHT). Adipose tissue samples were obtained in gonadectomized animals treated with vehicle (control group), or injected with 0.1 mg DHT 1, 3, 6, 12, 18, and 24 h prior to necropsy. Fourteen animals were pooled at each time point (total 196 animals). Transcripts that were significantly modulated were considered as androgen-responsive genes. Quantitative real-time RT-PCR was used to confirm results from the microarray analysis in a subset of 46 probe sets in male mice and 98 probe sets in female mice. Considering peak time vs. control, we confirmed 74.0 and 63.3% of the modulated genes by PCR in males and females, respectively. Four genes were significantly stimulated in a similar manner by DHT in both sexes, namely metallothionein 1, growth arrest and DNA-damage-inducible 45 gamma, cyclin-dependent kinase inhibitor 1A, and fk506-binding protein 5. All these genes appear to be involved in the regulation of adipocyte differentiation/proliferation and adipogenesis. In ...Continue Reading

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Citations

Apr 18, 2009·Future Cardiology·James P SticeAnne A Knowlton
May 18, 2012·Endocrine Research·Maya Otto-DuesselJeremy O Jones
Mar 9, 2010·The Journal of Steroid Biochemistry and Molecular Biology·Maria Rita De GiorgioJonny St-Amand
Sep 3, 2014·Molecular and Cellular Endocrinology·Marina NikolićDanijela Vojnović Milutinović
Mar 11, 2016·Endocrinology·Ioannis SimitsidellisPhilippa Tk Saunders
Mar 24, 2020·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Andrea Di NisioCarlo Foresta

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