PMID: 7513794May 1, 1994Paper

Response of the Muta mouse lacZ/galE- transgenic mutation assay to DMN: comparisons with the corresponding Big Blue (lacI) responses

Mutation Research
H TinwellJ Ashby

Abstract

The lacZ Muta mouse transgenic mutation assay was recently adapted into a selective assay based on use of E. coli galE(-) bacteria and phenyl galactoside (p-gal). A preliminary assessment of this selective assay was undertaken using a single oral dose of 10 mg/kg of dimethyl nitrosamine (DMN). The livers of treated male mice were assessed for UDS 2 h after dosing, and for lacZ- mutations 7, 11 and 20 days after dosing. A strong UDS response was recorded and a clear mutagenic response was observed at each of the 3 timepoints. Comparison of these data with earlier data derived using the Big Blue (lacI) mutation assay reveals a marginally greater sensitivity to DMN of the selective Muta mouse assay, an effect probably related to a biochemical difference between the strains of animal, as evidenced by the larger UDS response seen in the Muta mouse system. The original Muta mouse assay protocol was impractical. The galE- adaption makes the assay emminently practical and cost-effective. We are continuing to assess the true role of both the Big Blue assay and the galE- Muta mouse assay in mutagenicity/carcinogenicity prediction. The former assay enables access to B6C3F1 mice and F344 rats, the latter enables the rapid acquisition of data.

References

Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S W KohlerJ M Short
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J A GossenJ Vijg
Dec 1, 1987·Mutation Research·P H LohmanF Berends
Apr 1, 1985·Mutation Research·J AshbyM G Penman

❮ Previous
Next ❯

Citations

Oct 1, 1995·Mutation Research·X B ZhangJ A Heddle
Oct 22, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jason H Bielas, John A Heddle
May 1, 2001·Current Protocols in Toxicology·W C Ladiges, C B Ware
Jun 3, 2000·Japanese Journal of Cancer Research : Gann·Y ShioyamaM Katsuki
Jan 1, 1995·Environmental and Molecular Mutagenesis·N J Gorelick
Aug 6, 2005·Mutation Research·Iain B LambertGeorge R Douglas

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.