Response of the Pseudomonas host chromosomal transcriptome to carriage of the IncP-7 plasmid pCAR1

Environmental Microbiology
Masaki ShintaniHideaki Nojiri

Abstract

Plasmid carriage requires appropriate expression of the genes on the plasmid or host chromosome through cooperative transcriptional regulation. To clarify the impact of plasmid carriage on the host chromosome, we compared the chromosomal RNA maps of plasmid-free and plasmid-containing host strains using the incompatibility group P-7 archetype plasmid pCAR1, which is involved in carbazole degradation, and three distinct Pseudomonas strains. The possession of pCAR1 altered gene expression related to the iron acquisition systems in each host. Expression of the major siderophore pyoverdine was greater in plasmid-containing P. putida KT2440 and P. aeruginosa PAO1 than in the plasmid-free host strains, in part due to the expression of carbazole-degradative genes on pCAR1. The mexEFoprN operon encoding an efflux pump of the resistance-nodulation-cell division family was specifically upregulated by the carriage of pCAR1 in P. putida KT2440, whereas the expression of orthologous genes in the other species remained unaltered. Induction of the mexEFoprN genes increased the resistance of pCAR1-containing KT2440 to chloramphenicol compared with pCAR1-free KT2440. Our findings indicate that the possession of pCAR1 altered the growth rate of ...Continue Reading

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Citations

May 28, 2013·Trends in Ecology & Evolution·David A Baltrus
Jul 23, 2014·PloS One·Kevin DoughertyDavid A Baltrus
Dec 17, 2015·Molecular Biology and Evolution·Wesley Loftie-EatonEva M Top
Feb 15, 2015·Applied and Environmental Microbiology·Chiho Suzuki-MinakuchiHideaki Nojiri
Jul 17, 2019·Environmental Microbiology Reports·Ainara LuqueCristina Madrid
Nov 30, 2019·Environmental Microbiology·Ludovic Vial, Florence Hommais
Sep 25, 2020·Journal of Bacteriology·Satoru SumiHideaki Takano
Jul 7, 2020·Frontiers in Microbiology·Masatoshi MiyakoshiMasataka Tsuda
Jun 25, 2015·Microbiology Spectrum·Ana SeguraJuan Luis Ramos
Sep 26, 2017·Microbiology Spectrum·Alvaro San Millan, R Craig MacLean

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