PMID: 9166734Jun 1, 1997Paper

Responses of Bergmann glia and granule neurons in situ to N-methyl-D-aspartate, norepinephrine, and high potassium

Journal of Neurochemistry
Y Shao, K D McCarthy

Abstract

To gain insight into neuronal-glial signaling in brain, cerebellar Bergmann glia and granule neurons were studied in acutely isolated slices with the aid of laser scanning confocal microscopy. Both Bergmann glia and granule neurons responded to N-methyl-D-aspartate (NMDA) with a rise in [Ca2+]i. However, the glial NMDA response was frequently inhibited by tetrodotoxin, suggesting that the response depended on neuronal action potentials, rather than on direct activation of NMDA receptors on the Bergmann glia. Further experiments demonstrated that the NMDA response in Bergmann glia was not inhibited by a combination of non-NMDA glutamate receptor blockers 6-cyano-7-nitroquinoxaline-2,3-dione and alpha-methyl-4-carboxyphenylglycine. Bergmann glia also responded to norepinephrine and high K+, and the responses were not inhibited by tetrodotoxin. The glial norepinephrine response was blocked by phentolamine but not by the removal of external Ca2+, indicating a direct activation of alpha1-adrenergic receptors that mediated release of Ca2+ from intracellular stores. The KCl-induced response in both neurons and glia was dependent on external Ca2+ and was blocked by verapamil or nifedipine. In summary, our data indicate that Bergmann gl...Continue Reading

Citations

Feb 28, 2001·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·I PodkletnovaH Alho
Nov 23, 2007·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Monica Beneyto, James H Meador-Woodruff
May 10, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C G SchipkeF Kirchhoff
Oct 21, 2005·Journal of Neuroscience Research·Sabine HeroldJohannes Brockhaus
Sep 29, 2000·American Journal of Physiology. Heart and Circulatory Physiology·A BhardwajR C Koehler
Feb 22, 2001·Annual Review of Physiology·A AraqueP G Haydon
Mar 10, 1999·Methods : a Companion to Methods in Enzymology·L HertzJ C Lai
Aug 1, 1998·Brain Research. Molecular Brain Research·T LópezA Ortega
Aug 9, 2007·Brain Research Bulletin·Hugo Guerrero-CázaresSergio H Duenas-Jimenez

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