PMID: 15348799Sep 7, 2004Paper

Responses of bone to titania-hydroxyapatite composite and nacreous implants: a preliminary comparison by in situ hybridization

Journal of Materials Science. Materials in Medicine
H LiaoJ Li

Abstract

The effect of two biomaterials on bone formation in vivo by in situ hybridization, was compared by using RNA probes complementary to collagen alpha1(I) RNA, osteonectin RNA and osteocalcin RNA. Holes were drilled into the midshafts of rat femurs. Titania-hydroxyapatite composite (THA) or nacre cylinders were implanted and the bone-implant regions collected 14 days after operation. Cuboidal osteoblasts, intensely labelled with the three probes, were seen to be lining the newly formed bone surrounding the THA implant. Between the implant and the new bone, a layer of un-labelled, apparently non-osteogenic cells was observed. By contrast, the nacre implant was bonded to the newly formed bone without any soft tissue interference. Osteoblasts lining the distal surface of the newly formed bone were stained with all three RNA probes, although weaker than in the THA sample. Some of the osteoblasts were flattened. We concluded from the appearance of the osteoblasts that the bone formation in the nacre samples had progressed beyond the phase of maximal synthetic activity. Around the THA implant, the labelling indicated that bone-forming activity was still high. It was concluded that the bioactivity of nacre was higher than that of THA.

Citations

May 28, 2010·Journal of the Royal Society, Interface·A R BoccacciniI Zhitomirsky
Oct 18, 2016·Journal of Biomedical Materials Research. Part a·Ganggang ZhangMarthe Rousseau
Mar 10, 2017·Acta Biomaterialia·Ethan Michael GerhardJian Yang
May 9, 2002·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Lucilia Pereira MourièsEvelyne Lopez
May 8, 2021·Frontiers in Bioengineering and Biotechnology·Jingying PeiPeilong Sun

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