Responses of cytochrome P-450 isozymes of rat lung to in vivo exposure to ozone

Toxicology Letters
Y Takahashi, T Miura

Abstract

The effects of 2 weeks exposures of rats to 0.2 ppm O3 on pulmonary cytochrome P-450 isozymes were investigated. Two main types of cytochrome P-450 isozymes (cytochromes P-450FI and P-450FII) were separated from lung microsomes of control rats on an anion exchange cellulose column. Antibody raised against cytochrome P-450b, which is the main isozyme of liver obtained from phenobarbital-treated rats, cross-reacted with pulmonary cytochrome P-450FII, but not with cytochrome P-450FI. Ozone exposures caused increases in the content of both cytochromes P-450FI and P-450FII to the same extent two times greater than those of control rats without changes in immunological properties of these isozymes. No pulmonary cytochrome P-450 isozyme other than cytochromes P-450FI and P-450FII was observed in eluate through anion exchange column chromatography. These results indicate that increases in the content of pulmonary cytochrome P-450 of rats exposed to O3 can be ascribed to increase in constitutive types of the isozymes, but not to induction of other types of isozyme.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1986·Archives of Biochemistry and Biophysics·J B FaganH V Gelboin
Apr 1, 1984·Environmental Health Perspectives·R M Philpot, B R Smith
Jan 1, 1984·Journal of Toxicology and Environmental Health·D B Menzel
Aug 21, 1980·Biochimica Et Biophysica Acta·C R WolfR M Philpot

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