Responses of fibroblasts to anchorage of dorsal extracellular matrix receptors

Proceedings of the National Academy of Sciences of the United States of America
Karen A BeningoYu-Li Wang

Abstract

Fibroblasts in 2D cultures differ dramatically in behavior from those in the 3D environment of a multicellular organism. However, the basis of this disparity is unknown. A key difference is the spatial arrangement of anchored extracellular matrix (ECM) receptors to the ventral surface in 2D cultures and throughout the entire surface in 3D cultures. Therefore, we asked whether changing the topography of ECM receptor anchorage alone could invoke a morphological response. By using polyacrylamide-based substrates to present anchored fibronectin or collagen on dorsal cell surfaces, we found that well spread fibroblasts in 2D cultures quickly changed into a bipolar or stellate morphology similar to fibroblasts in vivo. Cells in this environment lacked lamellipodia and large actin bundles and formed small focal adhesions only near focused sites of protrusion. These responses depend on substrate rigidity, calcium ion, and, likely, the calcium-dependent protease calpain. We suggest that fibroblasts respond to both spatial distribution and mechanical input of anchored ECM receptors. Changes in cell shape may in turn affect diverse cellular activities, including gene expression, growth, and differentiation, as shown in numerous previous s...Continue Reading

References

Jun 1, 1978·Nature·J Folkman, A Moscona
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·F M WattC H O'Neill
Sep 1, 1972·The Journal of Cell Biology·T Elsdale, J Bard
Jan 1, 1995·Annual Review of Cell and Developmental Biology·B M JockuschJ Winkler
Jan 1, 1996·Annual Review of Cell and Developmental Biology·K Burridge, M Chrzanowska-Wodnicka
Jan 31, 1998·The Journal of Biological Chemistry·Anna HuttenlocherA Rick Horwitz
Jun 5, 1998·Biomaterials·A Curtis, C Wilkinson
Aug 8, 1998·Trends in Cell Biology·D D Schlaepfer, T Hunter
Mar 30, 1999·Biophysical Journal·Micah Dembo, Y Wang
Jun 27, 2000·Biophysical Journal·C M LoY Wang
Aug 19, 2000·Cellular and Molecular Life Sciences : CMLS·P Friedl, E B Bröcker
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·M J BerridgeM D Bootman
Nov 27, 2001·Science·Edna CukiermanKenneth M Yamada
Feb 12, 2002·Nature Reviews. Molecular Cell Biology·Deepika Walpita, Elizabeth Hay
Feb 21, 2002·Trends in Cell Biology·Angela GladingAlan Wells
Apr 11, 2002·Nature Cell Biology·Martin A Schwartz, Mark H Ginsberg
Dec 13, 2002·Nature·Sandrine Etienne-Manneville, Alan Hall
May 14, 2003·Trends in Cell Biology·Frederick Grinnell
May 27, 2003·Journal of Cell Science·Karen L Schmeichel, M J Bissell
Jul 16, 2003·Biochemical and Biophysical Research Communications·Christopher S ChenDonald E Ingber
Jul 31, 2003·The Journal of Biological Chemistry·Leo S PriceJohn G Collard
Sep 12, 2003·American Journal of Physiology. Cell Physiology·Daniel A EmmertRobert B Wysolmerski
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Alexander BershadskyBenjamin Geiger

Citations

Mar 10, 2006·Annals of Biomedical Engineering·Nathan J SniadeckiChristopher S Chen
Jan 22, 2008·Annals of Biomedical Engineering·Sheng-Yang LeeHaw-Ming Huang
Oct 10, 2009·Annals of Biomedical Engineering·Stephanie Nemir, Jennifer L West
Mar 17, 2009·Biomedical Microdevices·Xulang ZhangL James Lee
Jan 21, 2009·Cancer Metastasis Reviews·Roberto BuccioneInmaculada Ayala
Dec 20, 2005·Journal of Materials Science. Materials in Medicine·M BitarV Salih
Feb 5, 2008·In Vitro Cellular & Developmental Biology. Animal·Michele L MarquetteMarguerite Sognier
Sep 14, 2011·Nano Letters·Nadine PerschmannJoachim P Spatz
Dec 20, 2008·Nature Materials·Samir Mitragotri, Joerg Lahann
Jul 16, 2009·Nature Reviews. Molecular Cell Biology·Ryan J PetrieKenneth M Yamada
Dec 23, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Kristopher E KubowViola Vogel
Dec 23, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Mirren CharnleyMatthias P Lutolf
Jul 5, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Mirren CharnleyMarcus Textor
Feb 9, 2011·Physical Biology·Casey M Kraning-RushCynthia A Reinhart-King
May 6, 2008·Tissue Engineering. Part B, Reviews·Megan E FrancisEric M Brey
Jul 3, 2008·Tissue Engineering. Part a·Ram I SharmaPrabhas V Moghe
Feb 7, 2009·Tissue Engineering. Part a·Gary A MonteiroDavid I Shreiber
Aug 19, 2005·Molecular Biology of the Cell·Hongmei Jiang, Frederick Grinnell
Mar 7, 2008·Molecular Biology of the Cell·Miguel Miron-MendozaFrederick Grinnell
Jun 19, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Eric M BalzerKonstantinos Konstantopoulos
May 13, 2010·Spine·Cao YangZhenfeng Duan
Feb 18, 2011·Journal of Cardiovascular Pharmacology·Stephan Rohr
Mar 20, 2008·Journal of the Royal Society, Interface·Monika RumplerPeter Fratzl
May 3, 2008·Genes & Development·Xiaoming ZhouStephen J Weiss
Nov 10, 2006·Journal of Biomedical Optics·Frank DuboisChristine Decaestecker
Mar 13, 2008·Science Signaling·Rebecca G Wells, Dennis E Discher
Nov 28, 2007·Annual Review of Pathology·Christy HebnerJayanta Debnath
Jan 25, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Anant ChopraJ Yasha Kresh
May 23, 2012·Cancer Discovery·Tsukasa ShibueRobert A Weinberg
Oct 9, 2008·Journal of Cell Science·Vishnu V UndyalaKaren A Beningo
Dec 22, 2010·Journal of Cell Science·Paul A Janmey, R Tyler Miller
Jul 17, 2012·Journal of Cell Science·Brendon M Baker, Christopher S Chen
Jun 6, 2014·PLoS Computational Biology·Xin TangTaher A Saif
Sep 2, 2010·PloS One·Joseph E OlberdingKrishna Garikipati
Jan 1, 2010·Biotechnology & Genetic Engineering Reviews·Francesco Pampaloni, Ernst H K Stelzer
Oct 31, 2014·Nature Reviews. Molecular Cell Biology·Guillaume Charras, Erik Sahai
Jun 5, 2012·Biointerphases·José Ballester-BeltránManuel Salmeron-Sanchez
Mar 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sangmyung RheeFrederick Grinnell
Jul 7, 2009·Annual Review of Cell and Developmental Biology·Frederick Grinnell, W Matthew Petroll
Sep 1, 2014·Toxicology and Applied Pharmacology·Claudia MontaniDiego Di Lorenzo
Apr 24, 2014·Cell Communication & Adhesion·Sarah C StapletonChristopher S Chen
Sep 15, 2010·Journal of Bioscience and Bioengineering·Kazuaki NakamuraAkito Tanoue
Sep 17, 2015·Journal of Biomedical Materials Research. Part a·Valentina Laghezza MasciAnna Maria Fausto
May 21, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·D BazouS K Jackson
Dec 8, 2010·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Josemaria PaternoGeoffrey C Gurtner
Mar 1, 2012·Biotechnology and Bioengineering·Claire L RossouwDalu T Mancama
Oct 15, 2013·Immunological Reviews·Mélina L HeuzéAna-Maria Lennon-Duménil
Apr 4, 2006·Biophysical Journal·Alice Nicolas, Samuel A Safran
Jun 9, 2015·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Nicholas S GreavesArdeshir Bayat
Oct 4, 2006·Biomaterials·Chih-Chi WangTai-Horng Young
Aug 22, 2006·Current Opinion in Cell Biology·Melinda LarsenKenneth M Yamada
Aug 26, 2006·Current Opinion in Cell Biology·Alexander BershadskyBenjamin Geiger
Sep 19, 2006·Experimental Cell Research·Areum KimW Matthew Petroll
Jun 23, 2006·The International Journal of Biochemistry & Cell Biology·Mario Gimona, Roberto Buccione
Mar 15, 2006·Trends in Cell Biology·Grégory Giannone, Michael P Sheetz
Oct 24, 2012·Journal of Biomedicine & Biotechnology·Qianchen GuoHuajian Zhao
Aug 23, 2005·Current Opinion in Cell Biology·Sharona Even-Ram, Kenneth M Yamada
Aug 2, 2005·Seminars in Cancer Biology·Anna BirgersdotterIngemar Ernberg
Mar 4, 2015·Cell Adhesion & Migration·Jiří Kanta
Oct 1, 2007·Cell Adhesion & Migration·Tatiana Smirnova, Jeffrey E Segall
Nov 5, 2013·Biotechnology Advances·Zhaohui Li, Zhanfeng Cui
Feb 3, 2009·Current Biology : CB·Robert S FischerClare M Waterman
Jan 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Renaud PoinclouxPhilippe Chavrier
Mar 31, 2015·Nanomedicine·José Ballester-BeltránManuel Salmeron-Sanchez
Aug 28, 2007·Cell·Kenneth M Yamada, Edna Cukierman
Nov 16, 2010·Stem Cell Research·Andrew B J ProwseTrent P Munro
Jun 20, 2006·Journal of Theoretical Biology·Zhaochun YangJames H-C Wang
Jul 4, 2013·Experimental Cell Research·Miguel Miron-MendozaW Matthew Petroll
Mar 1, 2015·Biochemical and Biophysical Research Communications·Anand Ramanathan, Nancy Karuri
Feb 6, 2007·Biochemical and Biophysical Research Communications·Maria BokhariStefan A Przyborski
Feb 14, 2012·Biophysical Journal·Ailey CrowDaniel A Fletcher
Apr 23, 2010·Biophysical Journal·Wenting Shih, Soichiro Yamada
Nov 3, 2015·Experimental Cell Research·Andrew D Doyle, Kenneth M Yamada
Jul 12, 2014·Tissue Engineering. Part B, Reviews·Lucas T VuPadmavathy Rajagopalan
Feb 14, 2016·Journal of Biomedical Materials Research. Part a·Kalpana S KattiDinesh R Katti
Jan 17, 2017·IEEE Transactions on Nanobioscience·Keith C Heyde, Warren C Ruder
Mar 17, 2017·Molecular Biology of the Cell·Lauren D'AngeloKenneth A Myers
Sep 21, 2005·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M I D RossiR Borojevic
Aug 8, 2012·Scientific Reports·Warren C RuderJames F Antaki
Sep 2, 2017·Biology Open·Nicole M Myer, Kenneth A Myers
Oct 18, 2013·Integrative Biology : Quantitative Biosciences From Nano to Macro·Yi-hsuan LeeKeng-hui Lin
Aug 8, 2007·Journal of Cellular Physiology·Allison L Berrier, Kenneth M Yamada
Jun 12, 2008·Birth Defects Research. Part C, Embryo Today : Reviews·Satoshi Kurosaka, Anna Kashina
Jul 21, 2010·Soft Matter·Kristiana Kandere-GrzybowskaBartosz A Grzybowski
Jan 31, 2006·Biotechnology and Bioengineering·Charu P Vepari, David L Kaplan
Dec 29, 2013·Cold Spring Harbor Protocols·Jim SwogerErnst H K Stelzer
Jun 6, 2020·Investigative Ophthalmology & Visual Science·Kaku ItohHiroshi Ohguro
Jul 1, 2020·Small·Isabel M BjørgeJoão F Mano
Oct 13, 2015·Particle & Particle Systems Characterization : Measurement and Description of Particle Properties and Behavior in Powders and Other Disperse Systems·Pranay AgarwalXiaoming He
Sep 30, 2020·Expert Opinion on Drug Discovery·Carley OrtChristopher Moraes
Nov 5, 2013·Physiology·Daniel J Tschumperlin
Dec 20, 2014·The Journal of Biological Chemistry·Jennet ToyjanovaChristian Franck
Dec 14, 2016·International Journal of Molecular Medicine·Xiaoqian ZhangLanjuan Li
Sep 24, 2020·BioMed Research International·Amr AlraiesRyan Moseley
Oct 19, 2017·Biophysical Journal·Harsha KitturDino Di Carlo
Jul 28, 2015·The Journal of the American Osteopathic Association·Manal Zein-Hammoud, Paul R Standley
May 14, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·John E Kirkwood, Gerald G Fuller
Oct 29, 2005·Journal of Cell Science·Miguel Vicente-ManzanaresA Rick Horwitz
Dec 3, 2010·Journal of Cell Science·Manuel Théry
Aug 6, 2008·Molecular Pharmaceutics·Jayme L HorningVinod Labhasetwar

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
R J Pelham, Y Wang
Current Opinion in Cell Biology
Edna CukiermanKenneth M Yamada
© 2021 Meta ULC. All rights reserved