Responses of Listeria monocytogenes to acid stress and glucose availability revealed by a novel combination of fluorescence microscopy and microelectrode ion-selective techniques.

Applied and Environmental Microbiology
Lana ShabalaT A McMeekin

Abstract

Fluorescence ratio imaging microscopy and microelectrode ion flux estimation techniques were combined to study mechanisms of pH homeostasis in Listeria monocytogenes subjected to acid stress at different levels of glucose availability. This novel combination provided a unique opportunity to measure changes in H(+) at either side of the bacterial membrane in real time and therefore to evaluate the rate of H(+) flux across the bacterial plasma membrane and its contribution to bacterial pH homeostasis. Responses were assessed at external pHs (pH(o)) between 3.0 and 6.0 for three levels of glucose (0, 1, and 10 mM) in the medium. Both the intracellular pH (pH(i)) and net H(+) fluxes were affected by the glucose concentration in the medium, with the highest absolute values corresponding to the highest glucose concentration. In the presence of glucose, the pH(i) remained above 7.0 within a pH(o) range of 4 to 6 and decreased below pH(o) 4. Above pH(o) 4, H(+) extrusion increased correspondingly, with the maximum value at pH(o) 5.5, and below pH(o) 4, a net H(+) influx was observed. Without glucose in the medium, the pH(i) decreased, and a net H(+) influx was observed below pH(o) 5.5. A high correlation (R = 0.75 to 0.92) between the ...Continue Reading

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Citations

Sep 12, 2002·International Journal of Food Microbiology·T A McMeekin, T Ross
May 7, 2003·Applied and Environmental Microbiology·Adriana FerreiraKathryn J Boor
Apr 7, 2006·Applied and Environmental Microbiology·Marcelo BonnetThomas J Montville
Sep 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Paul D Cotter, Colin Hill
Nov 26, 2010·International Journal of Food Microbiology·Charalambia-Eirini A BelessiPanagiotis N Skandamis
Sep 17, 2008·Environmental Microbiology·Lana ShabalaSergey Shabala
Jul 11, 2006·International Journal of Food Microbiology·A O Gill, R A Holley
May 24, 2006·International Journal of Food Microbiology·Lana ShabalaHenrik Siegumfeldt
Nov 30, 2013·Pharmaceuticals·Filomena NazzaroVincenzo De Feo
Jun 14, 2015·Applied and Environmental Microbiology·Jie RenYu-Feng Yao
Nov 23, 2005·Journal of Food Protection·Adam R BaumannHao Feng
May 19, 2020·Food Science and Biotechnology·Chun-Feng JiaBo-Lin Zhang
Aug 21, 2020·Pest Management Science·Michelle MakRobert Spooner-Hart
Jan 15, 2008·Chemical Reviews·Johan BobackaAndrzej Lewenstam

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