Dec 6, 1978

Responses of mammalian metaphase chromosomes to endonuclease digestion

Chromosoma
C G SahasrabuddheT C Hsu

Abstract

Digestion of fixed metaphase chromosomes by endonucleases (micrococcal nuclease and DNase II) under optimal digestion conditions followed by Giemsa staining produces sharp banding patterns identical to G-bands. In 3H-thymidine labeled, synchronized metaphase cells of the chinese hamster (CHO line), the band induction is accompanied by the removal of DNA. The single strand specific nuclease S1 and DNase I do not produce such banding patterns.

Mentioned in this Paper

Chinese Hamster
Chromosome Banding
Deoxyribonuclease I
Micrococcal Nuclease
Thymidine
Giemsa Stain Method
Endonuclease
SDS-PAGE
Mitotic Metaphase
Eutamias

About this Paper

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