PMID: 8594419Jul 19, 1995Paper

Responses of mouse fast and slow skeletal muscle to streptozotocin diabetes: myosin isoenzymes and phosphorous metabolites

Molecular and Cellular Biochemistry
J G Fewell, T S Moerland

Abstract

A condition similar to insulin-dependent diabetes mellitus (IDDM) was induced in male CD-1 mice by injection of streptozotocin (STZ). Five weeks after treatment, the fast-twitch extensor digitorum longus (EDL) and slow-twitch soleus (SOL) muscles were isolated for analysis. Phosphorous metabolites were quantified by 31P-NMR and HPLC, native myosin was characterized electrophoretically, and activities of metabolic enzymes were measured spectrophotometrically. Relative to control animals, STZ-diabetes resulted in a significant 32% decrease in the FM1 isoform of myosin in EDL and a 24% decrease in IM myosin of SOL. Mass-specific activities of phosphofructokinase, citrate synthase, and cytochrome oxidase were significantly lower in SOL from STZ-diabetic mice than in controls by 23, 18, and 36%, respectively. Intracellular ATP was significantly lower in SOL from STZ-diabetic mice than in controls (3.44 +/- 0.20 mumol g-1 wet weight vs. 4.61 +/- 0.20 mumol g-1, respectively), as was creatine phosphate (11.98 +/- 0.80 mumol g-1 wet weight vs. 14.22 +/- 0.44 mumol g-1). In contrast to results from SOL, there were no significant changes in phosphorus metabolites or enzyme activity in EDL. These results show that the effects of IDDM on l...Continue Reading

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Citations

Dec 23, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Jeffrey D OvertonStephen T Kinsey
Sep 25, 2003·Muscle & Nerve·Lisa A LesniewskiR B Armstrong
Feb 28, 2009·Journal of Applied Physiology·Matthew P KrauseThomas J Hawke
Aug 17, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·S HeJ B Lingrel
Sep 25, 2007·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·A VignaudA Ferry
Nov 10, 2011·American Journal of Physiology. Endocrinology and Metabolism·Juha J HulmiHeikki Kainulainen

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