PMID: 16632756Apr 25, 2006Paper

Responses of rat pulp cells to heat stress in vitro

Journal of Dental Research
T AmanoM Shimono

Abstract

Although heat stress can cause irritation in the dentin/pulp complex, little is known about the thermotolerance of pulp cells and their response to heat stress. We investigated cultured rat pulp cell responses to heat stress. Cells were subjected to a temperature of 42 degrees C for 30 minutes, and HSPs, alkaline phosphatase activity, and gap-junctional communication were determined at various time points. Although only low levels of HSP70 expression were detected before heat treatment, heat shock markedly induced HSP70 expression, with it gradually increasing at 1 hour after being heated. HSP25, however, showed no dramatic change. Gap junction protein connexin43 rapidly degraded after heat treatment, recovering to normal levels within the following 6 hours. Alkaline phosphatase activity decreased immediately after heat stress, recovering after 1 hour. These results indicate that dental pulp possesses protective factors, including HSPs, and that it can recover viability of intercellular communication and alkaline phosphatase activity after heat stress.

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Citations

Jan 5, 2016·Scientific Reports·Christine AustinManish Arora
Mar 23, 2010·Dental Materials : Official Publication of the Academy of Dental Materials·Min LinBo Feng Bai
Aug 8, 2013·Microscopy Research and Technique·Takashi MuramatsuMasaki Shimono
May 21, 2009·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Jae-Hoon Ji, Young-Joo Jang
Aug 12, 2015·Archives of Oral Biology·Canussanun JirachotikoonUreporn Kedjarune-Leggat
May 20, 2016·BioMed Research International·E ArmellinL Cerroni
Oct 12, 2013·Archives of Oral Biology·Takashi MuramatsuMasaki Shimono
Dec 9, 2014·Dental Materials : Official Publication of the Academy of Dental Materials·Patrício RunnaclesFrederick Allen Rueggeberg
Dec 2, 2014·Archives of Oral Biology·V A CentenoR V Gallará
Jun 1, 2011·Journal of Applied Oral Science : Revista FOB·Junya YanoMasamichi Terashita
Sep 7, 2017·The Journal of Advanced Prosthodontics·Philipp-Cornelius PottMichael Eisenburger

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