Abstract
Iron is an essential micronutrient for all eukaryotic organisms. However, the low solubility of ferric iron has tremendously increased the prevalence of iron deficiency anemia, especially in women and children, with dramatic consequences. Baker's yeast Saccharomyces cerevisiae is used as a model eukaryotic organism, a fermentative microorganism, and a feed supplement. In this report, we explore the genetic diversity of 123 wild and domestic strains of S. cerevisiae isolated from different geographical origins and sources to characterize how yeast cells respond to elevated iron concentrations in the environment. By using two different forms of iron, we selected and characterized both iron-sensitive and iron-resistant yeast strains. We observed that when the iron concentration in the medium increases, iron-sensitive strains accumulate iron more rapidly than iron-resistant isolates. We observed that, consistent with excess iron leading to oxidative stress, the redox state of iron-sensitive strains was more oxidized than that of iron-resistant strains. Growth assays in the presence of different oxidative reagents ruled out that this phenotype was due to alterations in the general oxidative stress protection machinery. It was notewo...Continue Reading
References
Mar 15, 1996·Science·R StearmanA Dancis
May 2, 1997·The Journal of Biological Chemistry·D DixD Eide
Nov 27, 1999·The Journal of Biological Chemistry·X Z ChenM A Hediger
Jun 8, 2001·The Journal of Biological Chemistry·L LiJ Kaplan
Jul 4, 2002·The Journal of Biological Chemistry·Brian M Waters, David J Eide
Sep 10, 2003·The Journal of Biological Chemistry·Valérie HaurieFrancis Sagliocco
Dec 12, 2003·Molecular Biology of the Cell·Minoo Shakoury-ElizehCaroline C Philpott
Jan 18, 2005·Cell·Sergi PuigDennis J Thiele
Jan 13, 2006·Analytical Biochemistry·Jordi TamaritJoaquim Ros
Aug 29, 2006·Bioresource Technology·Maja PasPeter Raspor
Dec 12, 2007·Molecular and Cellular Biology·Liangtao LiJerry Kaplan
Jul 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Frank WiensRainer Spanagel
Feb 13, 2009·Nature·Gianni LitiEdward J Louis
Aug 27, 2009·Chemical Reviews·Craig D Kaplan, Jerry Kaplan
Dec 1, 2010·The Journal of Biological Chemistry·Huilan LinJerry Kaplan
Jan 26, 2011·Molecular Ecology·Francisco A CubillosGianni Liti
Feb 7, 2012·Biochimica Et Biophysica Acta·Caroline C PhilpottAvery G Frey
Mar 6, 2012·PLoS Biology·Keiji Nishida, Pamela A Silver
May 23, 2012·Biochimica Et Biophysica Acta·Roland LillUlrich Mühlenhoff
Jul 10, 2012·Cell Metabolism·Yueh-Jung LeeGuillaume F Chanfreau
Dec 21, 2012·Biochemistry·Gregory P Holmes-HamptonPaul A Lindahl
Aug 22, 2013·Current Opinion in Microbiology·Caryn E Outten, Angela-Nadia Albetel
Oct 22, 2013·G3 : Genes - Genomes - Genetics·Hana N LeeRachel B Brem
Dec 19, 2013·Biochemistry·Jinkyu ParkPaul A Lindahl
May 3, 2014·Biochemistry·Allison CockrellPaul A Lindahl
Jun 25, 2014·Molecular and Cellular Biology·Nerea SanvisensSergi Puig
Aug 6, 2014·The EMBO Journal·Fabio GsallerHubertus Haas
Aug 4, 2015·Nutrition·Mohammad Aref KyyalyElshahat Ramadan
Citations
Jun 19, 2018·PLoS Genetics·Lucía Ramos-AlonsoMaría Teresa Martínez-Pastor
Aug 24, 2018·Metallomics : Integrated Biometal Science·Lucía Ramos-AlonsoSergi Puig
Mar 13, 2016·Applied and Environmental Microbiology·Rosa de LlanosSergi Puig
Mar 21, 2017·World Journal of Microbiology & Biotechnology·María Teresa Martínez-PastorSergi Puig
Jun 6, 2018·Frontiers in Microbiology·Mikhail A EldarovAndrey V Mardanov
Oct 6, 2020·Frontiers in Microbiology·Lucía Ramos-AlonsoSergi Puig
Jun 15, 2019·Journal of Fungi·Peter J Brechting, Chad A Rappleye
Jul 3, 2021·Biomolecules·Karolina NowosadMarta Arczewska
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gordon A WalkerRon C Runnebaum
Aug 28, 2021·Genes·Raquel Sorribes-DaudenSergi Puig
Dec 16, 2020·Metallomics : Integrated Biometal Science·Antônio Jesus Dorighetto CogoAnna L Okorokova-Façanha