PMID: 2507512Sep 1, 1989Paper

Responses of type II pneumocyte antioxidant enzymes to normoxic and hyperoxic culture

In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association
P C PanusB A Freeman

Abstract

Cultured type II pneumocyte responses to in vitro normoxia (95% air:5% CO2) or hyperoxia (95% O2:5% CO2) were quantified. Normoxic culture (0 to 96 h) of rabbit type II cells resulted in enhanced cell-monolayer protein and DNA content. During this same time, cellular activities of superoxide dismutase (SOD), catalase, and glutathione peroxidase (GSH Px) decreased. Compared to cultures maintained in normoxia, hyperoxic exposure of cultures resulted in decreased cell-associated protein and DNA content. Exposure to hyperoxia also resulted in cytotoxicity as demonstrated by elevated cellular release of DNA, lactate dehydrogenase (LDH), and preincorporated 8-[14 C]adenine. Cellular catalase and GSH Px activities in hyperoxic cells decreased similarly to normoxic controls. In contrast, cellular SOD activity in hyperoxic cells decreased less than in normoxic cultures. Cellular SOD activity in hyperoxic cultures, when normalized for cellular protein, but not DNA, was greater than normoxic values after 24 to 96 h of exposure. Unlike the decrease in cellular antioxidant enzymes during normoxic and hyperoxic culture, cellular LDH activity increased during both these exposures. Cellular LDH activity in 24 to 96 h hyperoxia-exposed cells in...Continue Reading

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Citations

Oct 1, 1994·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·T OvesenO Elbrønd
Aug 12, 1998·American Journal of Respiratory Cell and Molecular Biology·P Pietarinen-RunttiV L Kinnula
Nov 1, 1990·American Journal of Respiratory Cell and Molecular Biology·C M HartE R Block
Jan 1, 1992·American Journal of Respiratory Cell and Molecular Biology·L Jornot, A F Junod
Jun 1, 1992·American Journal of Respiratory Cell and Molecular Biology·K KobayashiA Wanner
Jan 15, 2005·Free Radical Biology & Medicine·Carol A BallingerEdward M Postlethwait
Feb 19, 1991·Biochimica Et Biophysica Acta·P C PanusB A Freeman
Jan 1, 1997·Experimental Lung Research·S Y GardnerJ I Everitt
May 1, 1992·Archives of Biochemistry and Biophysics·P C PanusB A Freeman
Jul 9, 1999·Environmental and Molecular Mutagenesis·W J MeehanG K Ostrander

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