Restoration of Arpin suppresses aggressive phenotype of breast cancer cells

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Yi LiXuexia Zhang

Abstract

Arpin, a negative regulator of the actin-related protein-2/3 (Arp2/3) complex, is downregulated and predicts poor prognosis in breast cancer patients. However, its biological relevance in breast cancer is still unclear. This study was conducted to investigate the roles of Arpin in breast cancer growth and invasion. We overexpressed Arpin expression in MCF-7 and MDA-MB-231 breast cancer cells and examined the effects of restoration of Arpin on cell proliferation, colony formation, cell cycle distribution, invasion in vitro and tumorigenesis in vivo. The related molecular mechanism(s) was determined. It was found that ectopic expression of Arpin significantly decreased cell proliferation, colony formation, and tumorigenicity. Flow cytometric analysis showed that overexpression of Arpin significantly increased the percentage of G0/G1-phase cells and decreased the percentage of S-phase cells. Moreover, restoration of Arpin impaired the invasiveness of breast cancer cells, as determined by Transwell invasion assays. Mechanistically, overexpression of Arpin inhibited the phosphorylation of Akt in breast cancer cells. Co-expression of a constitutively active form of Akt blunted the suppression of cell proliferation and invasion by Arp...Continue Reading

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Citations

May 11, 2019·Cellular and Molecular Life Sciences : CMLS·Sandra Chánez-ParedesMichael Schnoor
May 21, 2021·Frontiers in Cell and Developmental Biology·Sandra Chánez-ParedesMichael Schnoor

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