Restoration of fibroblast growth factor receptor2 suppresses growth and tumorigenicity of malignant human prostate carcinoma PC-3 cells

The Prostate
Hiroaki YasumotoW L McKeehan

Abstract

Fibroblast growth factors (FGFs) and their receptors (FGFRs) expedite stromal-epithelial communication in development and homeostasis of the human prostate. Loss of resident epithelial cell FGFR2IIIb that responds to stromal FGF7 and FGF10 accompanies malignant progression of both model animal and human prostate tumors. We examined whether restoration of FGFR2IIIb by transfection in the malignant human prostate tumor PC-3 cell line restored cellular properties associated with less malignant tumors. Cell proliferation, apoptosis, and tumor cell implants were used to monitor malignant properties. Activity of FGFR2IIIb was assessed by immunoblot of FRS2 and p44/42 MAP kinase. Immunochemical analysis of pancytokeratin and lactoferrin expression was utilized to assess changes in cellular differentiation. Expression of FGFR2IIIb in PC-3 cells by transfection resulted in growth suppression in vitro and reduced tumor formation in vivo concurrent with increased cellular differentiation and apoptosis. The results indicate that restoration of FGFR2IIIb to the malignant human prostate epithelial cell prototype PC-3 restores properties associated with nonmalignant tumors and normal cells. This further suggests that epithelial cell resident,...Continue Reading

References

Jan 1, 1981·Urological Research·M van Sande, K Van Camp
Jan 1, 1984·Virchows Archiv. A, Pathological Anatomy and Histopathology·G Barresi, G Tuccari
Apr 23, 1999·The Journal of Biological Chemistry·W LuW L McKeehan
Jul 26, 2000·International Journal of Urology : Official Journal of the Japanese Urological Association·S NakataH Yamanaka
Sep 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y ZhangT Okamoto
Feb 6, 2003·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun
Jun 26, 2003·The New England Journal of Medicine·Peter T Scardino
Aug 20, 2003·International Journal of Cancer. Journal International Du Cancer·Gerald R CunhaWilliam A Ricke
Oct 1, 2003·The Prostate·Adrie van BokhovenM Scott Lucia

❮ Previous
Next ❯

Citations

Jan 18, 2006·Nucleic Acids Research·Ruben H HovhannisyanRuss P Carstens
Nov 3, 2010·Genes & Development·Charles J David, James L Manley
Feb 2, 2006·Molecular and Cellular Biology·Andrew P BaraniakMariano A Garcia-Blanco
Dec 24, 2005·Expert Opinion on Drug Delivery·Sergey A Kaliberov, Donald J Buchsbaum
Feb 19, 2016·Epigenetics : Official Journal of the DNA Methylation Society·Yu WuMin Fang
Mar 14, 2007·Cancer Genetics and Cytogenetics·Paula LázcozJavier S Castresana
Jul 4, 2007·The Journal of Pathology·K SahadevanV J Gnanapragasam
Aug 19, 2007·Differentiation; Research in Biological Diversity·Christine L ChafferElizabeth D Williams
Mar 13, 2012·International Immunopharmacology·Liuqin YangDianchun Fang
Dec 14, 2007·Molecular & Cellular Proteomics : MCP·Barry S TaylorArun Sreekumar
Feb 3, 2005·The Journal of Biological Chemistry·Eric J WagnerMariano A Garcia-Blanco
Feb 6, 2019·Journal of Clinical Medicine·Jun TeishimaAkio Matsubara
Oct 20, 2006·Critical Reviews in Clinical Laboratory Sciences·Edgardo V AriztiaDavid A Fishman
Jun 22, 2006·Journal of the National Cancer Institute·Paul W Finch, Jeffrey S Rubin
Jan 17, 2009·Molecular Cancer Research : MCR·Michael G GartsidePamela M Pollock
Nov 5, 2010·Molecular Cancer Research : MCR·Ellen Margrethe HaugstenJørgen Wesche

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Signaling by Tyrosine Kinases

Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones. RTKs have been shown not only to be key regulators of normal cellular processes but also to have a critical role in the development and progression of many types of cancer. Discover the latest research on cell signaling and RTK here.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis