PMID: 9188575Jul 1, 1997Paper

Restoration of interferon responses of adenovirus E1A-expressing HT1080 cell lines by overexpression of p48 protein

Journal of Virology
G T Leonard, G C Sen

Abstract

We have previously shown that both alpha interferon (IFN-alpha) and IFN-gamma signaling pathways are blocked in HeLa cells expressing the adenovirus E1A proteins (G. T. Leonard and G. C. Sen, Virology 224:25-33, 1996). Here, we report that in two other E1A-expressing cell lines derived from the HT1080 cells, neither IFN-alpha nor IFN-gamma could induce the transcription of genes containing the IFN-stimulated response element (ISRE). In contrast, IFN-gamma-mediated signaling to the gamma-activated sequence was unimpaired in these cells. This dichotomy was due to a lowered level of functional p48 protein but not of STAT1 protein in the E1A-expressing HT1080 cells. When p48 was overexpressed in those cells by stably transfecting a p48 expression vector, both types of IFN could effectively induce the transcription of ISRE-driven genes. Consequently, IFN-alpha was highly effective in inhibiting the replication of encephelomyocarditis virus in the E1A-expressing cells, which also overexpressed p48. These results reinforce the general conclusion that adenovirus E1A proteins block IFN signaling pathways by lowering the functional levels of one or more components of the trans-acting complexes that activate the transcription of IFN-stimu...Continue Reading

References

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Citations

Sep 27, 2003·Journal of Virology·Hongxing ZhaoCatharina Svensson
Nov 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jorge L Muñoz-JordanAdolfo García-Sastre
Aug 24, 2004·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Daniel T W ClarkeNigel A J McMillan
Jul 10, 1999·Immunological Reviews·J A Mahr, L R Gooding
Sep 7, 2001·Annual Review of Microbiology·G C Sen
Oct 5, 2001·Clinical Microbiology Reviews·C E Samuel
Nov 25, 2004·The Journal of Biological Chemistry·Daniel J HuiGanes C Sen
Mar 14, 1998·Proceedings of the National Academy of Sciences of the United States of America·X WeihuaD V Kalvakolanu
Jun 3, 2000·Annual Review of Immunology·D TortorellaH L Ploegh
Oct 6, 1998·Annual Review of Biochemistry·G R StarkR D Schreiber
Oct 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·L TerstegenL Graeve
Sep 16, 2003·The Journal of Biological Chemistry·Lidija KlampferLeonard Augenlicht
Nov 1, 2002·Reviews in Medical Virology·Bin GotohJunko Yokoo
Dec 19, 2002·International Reviews of Immunology·Christopher F Basler, Adolfo García-Sastre
Jun 8, 2007·Journal of Virology·Fulvia TerenziGanes C Sen
Jul 6, 2014·Avian Pathology : Journal of the W.V.P.A·István MészárosZoltán Zádori
Oct 20, 2000·The Journal of General Virology·W C Russell
Apr 6, 2001·Seminars in Immunology·A Abendroth, A M Arvin
Jan 4, 2001·Microbes and Infection·D R TaylorM M Lai

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