Restoration of miR-143 expression could inhibit migration and growth of MDA-MB-468 cells through down-regulating the expression of invasion-related factors

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Fatemeh TavanafarBehzad Baradaran

Abstract

Breast adenocarcinoma is the second common cancer in women the incidence of which is increasing in many countries, especially in developing companies. In this study, miRNA143 has been replaced by vector based microRNA-143 in breast adenocarcinoma cells (MDA-MB-468) and its anti-cancer effects on breast adenocarcinoma cells have been evaluated. The pCMV-MIR-143 vector was transfected into MDA-MB-468 cells via JetPEI transfection reagent. The transfected cells were selected by IC50 concentration of Geneticin antibiotic (G418) after a 2-week treatment. To evaluate the effect of miR-143 on the inhibition of migration, scratch wound healing assay was performed. Then, the expression level of miR-143, Kras, Vimentin, CXCR4, MMP9 and E-Cadherin were measured by the qRT-PCR method. Results of MTT and wound healing assays showed that miR-143 inhibited cell growth and cell migration in miR-143 induced cell line compared with control group. The result of gene expression showed that miR-143 reduced Kras, Vimentin, CXCR4 and MMP9 expression, and increased E-Cadherin expression in miR-143 replaced cells compared to control cells. The results showed that miRNA-143 plays an important role in cell growth and migration during breast cancer develo...Continue Reading

Citations

Jan 4, 2019·Journal of Cellular Physiology·Homa MollaeiZeinab Rostami
May 28, 2019·Journal of Cellular Physiology·Alireza AnvarniaDariush Shanehbandi
Jul 28, 2019·Pathogens and Disease·Narges DastmalchiSeyed Mahdi Banan Khojasteh
Aug 14, 2019·Journal of Cellular Biochemistry·Sheng YinMin Wang
Apr 30, 2019·Journal of Cellular Physiology·Leila KarimiDariush Shanehbandi
Feb 23, 2020·Cancer Science·Yoshihisa TokumaruYukihiro Akao
Jun 28, 2018·Breast Cancer Research and Treatment·Edna Ayerim Mandujano-TinocoVilma Maldonado
Jul 17, 2020·Non-coding RNA·Jun Sheng Wong, Yoke Kqueen Cheah
Dec 12, 2018·Journal of Cellular Biochemistry·Navid ShomaliBehzad Baradaran
Dec 17, 2020·Cell Biology International·Narges DastmalchiReza Safaralizadeh
Feb 12, 2021·Reproductive Sciences·Yalda Baghay EsfandyariAhad Mokhtarzadeh
Nov 6, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Tahereh ZeinaliBehzad Baradaran
Jul 3, 2021·International Journal of Molecular Sciences·Adriana FodorAngela Cozma
Jan 6, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Teng MaYongfeng Shao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Breast Invasive Carcinoma (Keystone)

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Breast Invasive Carcinoma

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.