Restored gap junctional communication in non-tumorigenic HeLa-normal human fibroblast hybrids

Carcinogenesis
H L de Feijter-RuppJ E Trosko

Abstract

Gap junctional intercellular communication (GJIC) has been implicated in homeostasis, development, differentiation, wound healing or regeneration and adaptive responses of differentiated cells. The dysfunction of homologous or heterologous GJIC has been associated with the tumorigenic phenotype. Restoration of growth control and the suppression of the tumorigenic phenotype have been previously associated with the up-regulation of GJIC by various anti-tumorigenic chemicals or transfection of connexin genes into tumor cells. To test the hypothesis that 'tumor suppressor' genes may be associated with the up-regulation of GJIC, we tested clones of tumorigenic HeLa, several non-tumorigenic HeLa-normal human fibroblast somatic cell hybrids and a tumorigenic segregant of one of the non-tumorigenic hybrids for GJIC. The parental HeLa cells (D98 AH.2) had no detectable GJIC but expressed detectable connexin 43 transcripts, while the non-tumorigenic HeLa-human fibroblast hybrids, which contained the chromosome 11 from the normal human fibroblast (CGL-1, CGL-2, ESH15 and EHS15c1), expressed ample connexin 43 transcripts and showed proficient GJIC. The tumorigenic segregant (CGL-3) from the non-tumorigenic HeLa-human fibroblast hybrid show...Continue Reading

Citations

Dec 18, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Kyung-Sun Kang, James E Trosko
Dec 29, 2011·Journal of Allergy·Steven R White
Jul 21, 2010·Journal of Comparative Pathology·A CorteggioG Borzacchiello
Dec 18, 2008·The Journal of General Virology·Jeroen WitteveldtArvind H Patel
Feb 15, 2000·Methods : a Companion to Methods in Enzymology·J E TroskoM Wade

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