Restoring the biophysical properties of decellularized patches through recellularization

Biomaterials Science
Gigi Chi Ting Au-YeungSubbu S Venkatraman

Abstract

Various extracellular matrix (ECM) scaffolds, isolated through decellularization, were suggested as ideal biomimetic materials for 'Functional tissue engineering' (FTE). The decellularization process comprises a compromise between damaging and preserving the ultrastructure and composition of ECM-previously shown to affect cell survival, proliferation, migration, organization, differentiation and maturation. Inversely, the effects of cells on the ECM constructs' biophysical properties, under physiological-like conditions, remain still largely unknown. We hypothesized that by re-cellularizing porcine cardiac ECM (pcECM, as a model scaffold) some of the original biophysical properties of the myocardial tissue can be restored, which are related to the scaffold's surface and the bulk modifications consequent to cellularization. We performed a systematic biophysical assessment of pcECM scaffolds seeded with human mesenchymal stem cells (MSCs), a common multipotent cell source in cardiac regenerative medicine. We report a new type of FTE study in which cell interactions with a composite-scaffold were evaluated from the perspective of their contribution to the biophysical properties of the construct surface (FTIR, WETSEM™) and bulk (DS...Continue Reading

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Citations

May 23, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Naoto WatanabeIchiro Sekiya
Dec 19, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Qing YaoYing-Zheng Zhao
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Mar 11, 2020·Polymers·Andrea Catalina Villamil BallesterosYuli Alexandra Neira Bejarano
Jun 24, 2021·BioMed Research International·Ryan A Behmer HansenSamuel E Senyo

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