Restraint Stress Induced Hyperpermeability and Damage of the Blood-Brain Barrier in the Amygdala of Adult Rats

Frontiers in Molecular Neuroscience
Guangming XuBin Cong

Abstract

Intense or prolonged exposure to stress can damage various brain structures, including the amygdala and hippocampus, which are associated with emotional-cognitive functions. Furthermore, this deterioration has been linked to a myriad of neurodegenerative and psychiatric disorders, in particular through disruption of the blood-brain barrier (BBB). However, insights remain scarce concerning the effects and mechanisms associated with stress on the BBB in the amygdala. This study explored the effects of restraint stress on the permeability and integrity of the BBB in the amygdala of male adult SD rats. Serum levels of corticosterone (CORT) and S100B were determined through ELISA. The permeability of the BBB was assessed by measuring Evans Blue (EB) leakage in tissue samples from the rats' amygdala. These samples were immunostained for markers of tight junctions (Claudin-5, Occludin, ZO-1) and adherens junctions (VE-cadherin), as well as GLUT-1 and AQP-4. Staining was evaluated through confocal microscopy, and the level of expression of these proteins was quantified using the Western Blot (WB) technique. The ultrastructure of brain microvascular endothelial cells was assessed with transmission electron microscopy. Moreover, interleu...Continue Reading

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Citations

Aug 20, 2019·The European Journal of Neuroscience·Katarzyna A DudekCaroline Menard
Jul 28, 2019·Frontiers in Neuroendocrinology·Josep Moreno-Rius
Jan 27, 2021·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Peter KarailievDaniela Jezova
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Sep 3, 2021·Brain, Behavior, and Immunity·Oktay GenelAlessandra Borsini
Oct 13, 2021·Cellular and Molecular Neurobiology·Shusheng WuLongfei Du

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Methods Mentioned

BETA
ELISA
Protein Assay
electrophoresis
transmission electron microscopy
Transmission
electron microscopy

Software Mentioned

SPSS
Leica Application Suite X
Leica Application Suite
Leica TCS SP8
ImageJ

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