Restricted cytosolic growth of Francisella tularensis subsp. tularensis by IFN-gamma activation of macrophages.

Microbiology
Jessica A EdwardsJean Celli

Abstract

The intracellular bacterium Francisella tularensis ensures its survival and proliferation within phagocytes of the infected host through phagosomal escape and cytosolic replication, to cause the disease tularemia. The cytokine interferon-gamma (IFN-gamma) is important in controlling primary infections in vivo, and in vitro intracellular proliferation of Francisella in macrophages, but its actual effects on the intracellular cycle of the bacterium are ambiguous. Here, we have performed an extensive analysis of the intracellular fate of the virulent F. tularensis subsp. tularensis strain Schu S4 in primary IFN-gamma-activated murine and human macrophages to understand how this cytokine controls Francisella proliferation. In both murine bone marrow-derived macrophages (muBMMs) and human blood monocyte-derived macrophages (MDMs), IFN-gamma controlled bacterial proliferation. Schu S4 growth inhibition was not due to a defect in phagosomal escape, since bacteria disrupted their phagosomes with indistinguishable kinetics in both muBMMs and MDMs, regardless of their activation state. Rather, IFN-gamma activation restricted cytosolic replication of Schu S4 in a manner independent of reactive oxygen or nitrogen species. Hence, IFN-gamma ...Continue Reading

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Citations

Jun 13, 2012·Microbiology and Molecular Biology Reviews : MMBR·Crystal L JonesDavid S Weiss
Oct 22, 2014·Journal of Immunology Research·Julia A TreeCaroline A Rowland
Aug 30, 2014·Journal of Immunology Research·Sarah L NewsteadRoman A Lukaszewski
Aug 21, 2013·Virulence·Jerod A Skyberg
Mar 19, 2014·Frontiers in Cellular and Infection Microbiology·Bradley D JonesJoshua R Fletcher
Jan 8, 2015·Frontiers in Cellular and Infection Microbiology·Joseph J GillardCarmen Molina-París
May 2, 2014·Frontiers in Cellular and Infection Microbiology·Devyn D GilletteMikhail A Gavrilin
Jul 28, 2016·Journal of Molecular Biology·Cheryl Miller, Jean Celli
Sep 27, 2016·Diseases of Aquatic Organisms·Kathrine R BakkemoMarit Seppola
Mar 25, 2015·Infection and Immunity·Johan BinesseAnders Sjöstedt
Jun 18, 2014·Infection and Immunity·Marie LindgrenAnders Sjöstedt
Nov 17, 2016·Journal of Clinical Microbiology·Padmapriya P BanadaDavid Alland
Apr 24, 2018·Frontiers in Cellular and Infection Microbiology·Lydia M RobertsJeffrey A Frelinger
Jun 6, 2017·Nature Communications·Sivakumar PeriasamyJonathan A Harton

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