Restricted entry of an anti-rat epididymal protein IgG into the rat epididymis

International Journal of Andrology
P Y WongH K Lau

Abstract

A rat epididymal protein (MW 32000) was isolated and purified from the rat caudal epididymal fluid. Mono-specific antiserum against this protein was raised in rabbits, purified and labelled with 125I. The labelled IgG was infused intravenously into anaesthetized male rats, and the transfer of the labelled IgG across the luminally perfused cauda epididymidis was studied. It was found that during the 2 h infusion period, radioactivity in blood rose, but no radioactivity could be detected in the perfusates, irrespective of whether the epididymis was perfused with Krebs bicarbonate solution or a solution which resembled the rat caudal fluid in ionic composition. In some experiments, rats were given a single intravenous injection of labelled IgG and radioactivity in the epididymal content was measured 10 days later. It was found that despite a high IgG level in blood and liver, no radioactivity could be detected in epididymal fluid and sperm. It is concluded that the blood-epididymis barrier restricts the passage from blood to lumen, an immunoglobulin directed against an epididymal protein.

References

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Citations

Jun 1, 1987·Cell and Tissue Research·T G CooperM Bergmann
Feb 1, 1995·Journal of Reproductive Immunology·P Pöllänen, T G Cooper
Feb 1, 1995·Journal of Reproductive Immunology·P PöllänenT G Cooper
May 1, 1994·Journal of Reproductive Immunology·P Pöllänen, T G Cooper
Feb 1, 1997·Journal of Reproductive Immunology·P PöllänenB P Setchell
Oct 1, 1991·International Journal of Andrology·C H YeungT G Cooper
Jan 7, 2011·Biology of Reproduction·Payal MitalJannette M Dufour
Jan 1, 1991·Annals of the New York Academy of Sciences·T T Turner
Jun 19, 2002·American Journal of Reproductive Immunology : AJRI·M PomeringKenneth W Beagley
Nov 1, 1985·Journal of Andrology·P Esponda, J M Bedford

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