Restricted intra-embryonic origin of bona fide hematopoietic stem cells in the chicken

Development
Laurent Yvernogeau, Catherine Robin

Abstract

Hematopoietic stem cells (HSCs), which are responsible for blood cell production, are generated during embryonic development. Human and chicken embryos share features that position the chicken as a reliable and accessible alternative model to study developmental hematopoiesis. However, the existence of HSCs has never been formally proven in chicken embryos. Here, we have established a complete cartography and quantification of hematopoietic cells in the aorta during development. We demonstrate the existence of bona fide HSCs, originating from the chicken embryo aorta (and not the yolk sac, allantois or head), through an in vivo transplantation assay. Embryos transplanted in ovo with GFP embryonic tissues on the chorio-allantoic membrane provided multilineage reconstitution in adulthood. Historically, most breakthrough discoveries in the field of developmental hematopoiesis were first made in birds and later extended to mammals. Our study sheds new light on the avian model as a valuable system to study HSC production and regulation in vivo.

References

Jul 1, 1975·The Journal of Experimental Medicine·N M Le Douarin, F V Jotereau
Jul 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·N M Le DouarinM Belo
Oct 1, 1967·The Journal of Experimental Medicine·M A Moore, J J Owen
Jul 1, 1969·Toxicology and Applied Pharmacology·A I WalkerJ Robinson
Sep 2, 1967·Nature·M A Moore, J J Owen
Jan 1, 1980·Scandinavian Journal of Immunology·O LassilaF Dieterlen-Lièvre
Nov 1, 1981·Developmental Biology·F Dieterlen-Lièvre, C Martin
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A CumanoC J Paige
Nov 1, 1995·Anatomy and Embryology·J A Garcia-PorreroF Dieterlen-Lièvre
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·Q WangN A Speck
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·A CaprioliF Dieterlen-Lièvre
Aug 13, 1998·Developmental and Comparative Immunology·D DunonB A Imhof
Aug 6, 2000·Developmental Biology·T JaffredoF Dieterlen-Lièvre
Mar 17, 2004·PLoS Biology·Julie L ChristensenIrving L Weissman
Jun 12, 2004·EMBO Reports·Michael J McGrewHelen Sang
Mar 2, 2005·Developmental Cell·Christos GekasHanna K A Mikkola
Mar 2, 2005·Developmental Cell·Katrin Ottersbach, Elaine Dzierzak
Feb 17, 2006·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Karine BollerotThierry Jaffredo
Mar 1, 1917·Proceedings of the National Academy of Sciences of the United States of America·H E Jordan
May 23, 2006·Trends in Cardiovascular Medicine·Françoise Dieterlen-LièvreThierry Jaffredo
May 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·Samir Taoudi, Alexander Medvinsky
Dec 2, 2008·Cell Stem Cell·Ann C ZoveinM Luisa Iruela-Arispe
Feb 16, 2010·Nature·Jean-Charles BoissetCatherine Robin
Jan 7, 2011·Proceedings of the National Academy of Sciences of the United States of America·Momoko YoshimotoMervin C Yoder
Jun 1, 2011·The Journal of Experimental Medicine·Stanislav RybtsovAlexander Medvinsky
Nov 1, 2011·Nature Protocols·Jean-Charles BoissetCatherine Robin
Nov 2, 2011·The Journal of Experimental Medicine·Andrejs IvanovsAlexander Medvinsky
Dec 2, 2011·Development·Laurent YvernogeauJosiane Fontaine-Perus

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Citations

Nov 3, 2017·The Journal of Experimental Medicine·Alison C McGarveyAlexander Medvinsky
Dec 24, 2018·Nature Communications·Miguel GanuzaShannon McKinney-Freeman
Mar 28, 2019·Frontiers in Cell and Developmental Biology·Christopher B Mahony, Julien Y Bertrand
Dec 18, 2020·Frontiers in Cell and Developmental Biology·Pedro SecoAna Teresa Tavares
Jan 5, 2021·Frontiers in Cell and Developmental Biology·Ramy ElsaidAna Cumano
Feb 2, 2021·Poultry Science·E A Wong, Z Uni

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Methods Mentioned

BETA
transgenic
dissection
Flow cytometry
flow
PCR
FCS

Software Mentioned

Imaris
Zeiss Zen

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