Restricted use of T-cell receptor V beta genes in posttransfusion graft-versus-host disease

Transfusion
L WangT Juji

Abstract

Posttransfusion graft-versus-host disease (PT-GVHD) is a pathophysiological process of the immune system. Mature donor T cells that have survived the host defense mechanisms recognize recipient allo-antigens through antigen-specific T-cell receptors and mount an immune attack against certain host antigens. To characterize the T cells involved in PT-GVHD, expression of the T-cell receptor V beta genes in peripheral blood T cells isolated from four PT-GVHD patients was analyzed. T-cell receptor beta chain cDNA derived from mRNA extracted from peripheral blood lymphocytes was amplified by an inverse polymerase chain reaction method, and the cDNA was subsequently cloned and sequenced. The cDNA clones derived from patients with PT-GVHD showed remarkable differences in the distribution of T-cell receptor V beta gene use from that in normal controls. Very restricted T-cell receptor V beta gene repertoires and preferential expression of certain V beta gene segments were revealed in the peripheral blood lymphocytes of each patients. Shared V beta gene use among the patients was not observed, probably because of HLA differences among the patient-donor combinations. This restricted use reflected in vivo expansion of a limited number of en...Continue Reading

Citations

Dec 13, 2006·Clinical & Developmental Immunology·Nelson M VazClaudia R Carvalho
Mar 27, 2001·Biochemical and Biophysical Research Communications·M WakuiK Tokunaga
Apr 24, 2007·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Tutku OzdoğanElif Yildiz
May 8, 2009·Current Medical Research and Opinion·Michael J Cowley, Louis Kuritzky
Sep 24, 2004·Physiological Measurement·Hamid HassanpourBoualem Boashash
Jan 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christine L O'KeefeJaroslaw P Maciejewski

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

© 2021 Meta ULC. All rights reserved