Restricted utilization of VH and DH genes in leukemic rabbit B cells

European Journal of Immunology
R S BeckerK L Knight

Abstract

Polyclonal B cell leukemias have been generated in 17- to 20-day old Emu-myc transgenic rabbits. To analyze the repertoire of VH genes utilized in early B cells, eight VDJ genes were cloned from these leukemic cells. The nucleotide sequences of these genes indicated that seven of the eight VDJ genes encoded prototype VHa1, VHa2 or VHa3 allotypes. The two VDJ genes encoding VHa1 molecules had VH segments with identical nucleotide sequences; similarly, the VH segments of the four VDJ genes encoding VHa2 molecules were identical, with the exception of a single base pair. These data suggest that a limited repertoire of VH genes were utilized in the generation of these VDJ genes. The DH segments of these genes were limited to two DH families, D1 and D2, indicating that a restricted repertoire of DH genes also had been utilized. Since these leukemic cells probably developed early in ontogeny, we suggest that this restricted utilization of VH and DH genes is representative of B cells from developmentally immature rabbits.

References

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Citations

Apr 1, 1997·Current Opinion in Immunology·K L Knight, C R Winstead
Mar 27, 2012·Nature Biotechnology·Wan Cheung CheungRoberto D Polakiewicz
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·R PospisilR G Mage
Oct 1, 1996·The Journal of Experimental Medicine·R PospisilR G Mage
May 25, 1994·Nucleic Acids Research·D A RamsdenG E Wu
May 29, 1998·Immunological Reviews·K L Knight, R A Barrington
Nov 19, 2015·Developmental and Comparative Immunology·Domenick E KennedyKatherine L Knight
Nov 10, 2015·Developmental and Comparative Immunology·Domenick E KennedyKatherine L Knight
Jul 7, 2007·Veterinary Immunology and Immunopathology·Edita JeklovaMartin Faldyna
Nov 14, 2006·Veterinary Immunology and Immunopathology·Stephen M Wilson, Bruce N Wilkie
Jul 1, 1993·Research in Immunology·M A CraneK L Knight
Mar 25, 2017·Experimental & Molecular Medicine·Justus WeberChristoph Rader
Sep 29, 1995·Annals of the New York Academy of Sciences·K L Knight, M A Crane
Jan 1, 1997·International Reviews of Immunology·K L Knight, C R Winstead
Apr 8, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Li MaYaofeng Zhao
Jun 1, 1990·European Journal of Immunology·L A DiPietroK L Knight

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