Abstract
The nucleotide sequences of the variable region genes encoding five different human, high affinity antibodies, specific for the major neutralization determinant (AD-1) expressed by human cytomegalovirus glycoprotein B (gp58/116), have been determined. Three of the five heavy chain variable regions belonged to the small VHV-family, although they combined with a diverse set of light chains (V kappa IIIb, V lambda II and V lambda III). The other two antibodies belonged to VH-families III and IV. One of the VHV-family genes most likely originated from a previously unreported germline gene or allele, since it carries a nine nucleotide insert in framework 1. In addition, V lambda-genes showed variable homology (77-95%) to known germline sequences, while V kappa-genes showed high homology (approximately 98%) with their proposed germline origin. Despite the close homology of the V kappa IIIb-gene used to express one of the antibodies with its corresponding germline gene, the protein did not strongly express some idiotypes associated with this light chain family. There is, thus, no direct relation between the expression of these crossreactive idiotypes and the use of even modestly mutated light chains belonging to this V kappa-family, w...Continue Reading
References
Sep 1, 1992·The Journal of General Virology·H MeyerM Mach
Oct 5, 1992·Journal of Molecular Biology·I M TomlinsonG Winter
Dec 1, 1992·Molecular Immunology·M D DaleyK A Siminovitch
Feb 1, 1992·Molecular Immunology·M J CampbellR Levy
Sep 1, 1990·Molecular Immunology·V GuigouF Fumoux
Oct 1, 1990·The Journal of General Virology·H MeyerM Mach
May 1, 1991·European Journal of Immunology·M E SchutteT Logtenberg
Feb 1, 1991·The Journal of Experimental Medicine·M YamadaG Rovera
Jun 1, 1991·European Journal of Immunology·G Combriato, H G Klobeck
Jun 1, 1990·European Journal of Immunology·P ChuchanaM P Lefranc
Mar 1, 1990·The Journal of General Virology·H E Farrell, G R Shellam
Jul 3, 1990·Journal of Immunological Methods·D BronP Stryckmans
Aug 1, 1987·The Journal of Experimental Medicine·M M NewkirkJ D Capra
May 1, 1988·The Journal of Clinical Investigation·M M NewkirkR L Wasserman
May 25, 1989·Nucleic Acids Research·F BrocklyM P Lefranc
Nov 1, 1985·Annals of the Rheumatic Diseases·P J VenablesR N Maini
Feb 4, 1988·Nature·C G HumphriesP W Tucker
Feb 1, 1993·Molecular Immunology·M M NewkirkG J Silverman
Apr 1, 1993·The Journal of Experimental Medicine·L Z Rassenti, T J Kipps
Sep 17, 1993·Science·L BerberianJ Braun
Jul 1, 1993·European Journal of Immunology·S C Williams, G Winter
Aug 1, 1993·Molecular Immunology·S Avrameas, T Ternynck
Feb 1, 1993·Annals of Internal Medicine·J M GoodrichJ D Meyers
Feb 1, 1993·Annals of Internal Medicine·D J WinstonR E Champlin
May 30, 1993·Gene·M Ayala AvilaC A Borrebaeck
Citations
Nov 11, 1999·European Journal of Immunology·H A CurtisM M Newkirk
Jan 1, 1996·Molecular Immunology·M OhlinC A Borrebaeck
May 1, 1996·Molecular Immunology·M Ohlin, C A Borrebaeck
Feb 1, 1996·FEMS Immunology and Medical Microbiology·M Ohlin, C A Borrebaeck
Mar 30, 2002·Molecular Immunology·Christina FurebringMats Ohlin
Aug 12, 2003·Molecular Immunology·Mats Ohlin, Moncef Zouali
Sep 15, 1998·Molecular Immunology·M Ohlin, C A Borrebaeck
Aug 15, 1998·Vox Sanguinis·C V Prowse
Dec 1, 1995·European Journal of Biochemistry·T BöldickeW Lindenmaier
May 26, 2009·New Biotechnology·Fredrika AxelssonMats Ohlin
Mar 25, 2015·Molecular Immunology·Mats Ohlin, Cecilia Söderberg-Nauclér
Apr 5, 2001·Protein Engineering·P JirholtM Ohlin
Mar 1, 1996·European Journal of Immunology·A AyoubaA Coutinho
Mar 16, 2002·Blood·Delin ZhuFreda K Stevenson
Apr 15, 2020·The Journal of Infectious Diseases·Ayub AliOtto O Yang