PMID: 2856Dec 30, 1975

Restriction and modification in B. subtilis. Purification and general properties of a restriction endonuclease from strain R

Molecular & General Genetics : MGG
S BronT A Trautner

Abstract

All Bacillus subtilis R-type strains showing the phenomena of restriction and modification contain an endonuclease that inactivates in vitro the biological activity of a variety of DNAs lacking R-specific modification, such as transfecting SPPI, SPO2 and phi105 DNA, and transforming B. subtilis 168-type DNA. The corresponding DNAs carrying R-specific modification are resistant to the enzyme. The enzyme has been purified approximately 400-fold and is essentially free from contaminating double strand-directed unspecific exo- or endonuclease activity. Only Mg2+ is required as cofactor. The substrate DNAs are cleaved at specific sites. The double-stranded fragments produced from SPP1 DNA (molecular weight 2.5 x 10(7)) have an average molecular weight of about 3 x 10(5).

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Related Concepts

Striadyne
Natto Bacteria
Bacteriophages
Calcium
Coliphages
DNA Restriction Enzymes
DNA, Bacterial
DNA, Viral
Endonuclease
Hydrogen-Ion Concentration

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