PMID: 8441677Feb 11, 1993Paper

Restriction endonuclease cleavage of 5-methyl-deoxycytosine hemimethylated DNA at high enzyme-to-substrate ratios

Nucleic Acids Research
P S NelsonC W Schweinfest

Abstract

We have investigated the ability of a large number of restriction enzymes to digest non-canonically hemimethylated DNA at high enzyme-to-substrate ratios. A single-stranded unmethylated phagemid was used as a template to complete synthesis of the second strand using 5-methyl-dCTP to substitute for all the deoxycytosine residues. A fragment of this double-stranded hemimethylated DNA which contains the multiple cloning site region was used as a substrate. For all the enzymes tested, at least some degree of protection from digestion is observed. Sites completely protected from digestion by their cognate enzymes are SalI, BstXI, SacI, SacII, SmaI, SstI, XhoI, PstI, HinfI, BamHI and AccI. Sites partially protected from digestion by their cognate enzymes are XbaI, HindIII, KpnI, SpeI, ClaI, EcoRI and PvuII. Knowledge of the sensitivity of commonly used restriction enzymes to hemimethylated substrates is useful for several applications, which will be discussed.

References

Nov 1, 1976·Journal of Molecular Biology·G J RussellJ H Subak-Sharpe
Jan 1, 1991·Annual Review of Genetics·G G Wilson, N E Murray
Sep 25, 1987·Cell·D W Russell, N D Zinder
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
Nov 7, 1980·Science·A Razin, A D Riggs
Jun 19, 1981·Science·M Ehrlich, R Y Wang
Jun 11, 1981·Nucleic Acids Research·Y GruenbaumA Razin
Jun 25, 1983·Nucleic Acids Research·K A Armstrong, W R Bauer

❮ Previous
Next ❯

Citations

Mar 23, 2011·Biochemistry·Akiko Nomura, Akimitsu Okamoto
Nov 20, 2008·Cancer Science·Shintaro Shimamura, Fuyuki Ishikawa
Dec 17, 2008·Organic & Biomolecular Chemistry·Akimitsu Okamoto
May 8, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Toufic RennoJean-François Gauchat
Oct 27, 2001·Molecular Microbiology·C PossozM Guérineau
Sep 10, 2020·Molecular and Cellular Biology·Eriko WatadaTakehiko Kobayashi
Sep 17, 1999·Journal of Clinical Microbiology·E SeverinaA Tomasz
Jan 1, 1996·Journal of Bacteriology·D van SoolingenP W Hermans

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.