PMID: 1206800Jan 1, 1975Paper

Restriction enzyme digests of rapidly renaturing fragments of vaccinia virus DNA

Journal of Virology
F DeFilippes

Abstract

Vaccinia virus DNA fragments that have been denatured by alkali and then neutralized contain a fraction that rapidly reforms duplex structures. The fraction is enriched by fractionating on hydroxyapatite columns and serves as as substrate for digestion by two restriction endonucleases isolated from Hemophilus parainfluenzae, Hpa I and HPa II. The patterns obtained by gel electrophoresis of the digested fragments show the presence of three major bands after Hpa I digestion and four major bands after Hpa II digestion. The DNA that is isolated from some of these bands quickly reforms duplex regions after alkaline denaturation. The size of the DNA segments in the major bands has been estimated to be in the range of 0.44 X 10(6) to 3.2 X 10(6) daltons. The fragments which rapidly reform duplex chains after denaturation are sensitive to single-strand-specific nucleases. These results are consistent with a model of vaccinia virus DNA which has a covalent link connecting complementary chains.

References

Mar 25, 1974·Journal of Molecular Biology·D A Wilson, C A Thomas
Oct 1, 1968·Journal of Virology·B Moss, N P Salzman
Aug 5, 1973·Journal of Molecular Biology·K J DannaD Nathans
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·B E GriffinA Cowie
Oct 5, 1974·Journal of Molecular Biology·P Geshelin, K I Berns
Jun 4, 1974·Biochemical and Biophysical Research Communications·F M DeFilippes
Dec 21, 1973·Biochimica Et Biophysica Acta·D A Wilson, C A Thomas
Jul 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·B G Pogo, S Dales
Aug 16, 1965·Biochemical and Biophysical Research Communications·G FairbanksR H Reeder
Mar 1, 1970·Journal of Virology·K I Berns, C Silverman
Jun 15, 1961·Proceedings of the National Academy of Sciences of the United States of America·J MARMUR, L GROSSMAN

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Citations

Mar 15, 1982·Experientia·R Wittek
Oct 1, 1977·Journal of Virology·G Pedrali-Noy, A Weissbach
Jul 1, 1982·Journal of Virology·F M DeFilippes

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