Restriction fragment length polymorphism and spacer oligonucleotide typing: a comparative analysis of fingerprinting strategies for Mycobacterium bovis

Veterinary Microbiology
A AranazD Cousins

Abstract

The combination of conventional investigation and DNA fingerprinting is yielding important insights into the epidemiology of Mycobacterium bovis infections. Various genetic markers used in restriction fragment length polymorphism (RFLP) have recently been exploited for fingerprinting of M. bovis isolates. The newly developed spacer oligonucleotide typing aimed to investigate the polymorphism of M. tuberculosis in the DR locus, has also been applied to the molecular typing of M. bovis isolates. This work compared the performance of the insertion sequence (IS) IS6110, IS1081 and the genetic elements polymorphic G + C-rich repeat (PGRS) and direct repeat (DR) used in RFLP analysis with spoligotyping using a group of 128 Spanish M. bovis isolates. In this study, the most sensitive technique for identifying polymorphism in M. bovis was PGRS-RFLP, closely followed by IS6110-RFLP. We propose several schemes for fingerprinting of these isolates, however, the clear geographical variations found by different authors makes the study of each local situation indispensable. An international consensus in the methods used would be desirable for efficient interlaboratory comparison of strains.

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Citations

Nov 26, 2003·Veterinary Microbiology·A ParraJ Hermoso de Mendoza
Jan 11, 2001·FEMS Microbiology Letters·T G PattonS S Crupper
Apr 5, 2003·Applied and Environmental Microbiology·Luc BelloyJoachim Frey
Nov 26, 2010·Journal of Bacteriology·Stefan BergNoel H Smith
Jan 4, 2001·Journal of Clinical Microbiology·B M Njanpop-LafourcadeN Haddad
Sep 28, 2001·Journal of Clinical Microbiology·N HaddadB Durand
Jun 9, 2004·Journal of Clinical Microbiology·Alicia AranazLucas Domínguez
Oct 7, 2005·Journal of Clinical Microbiology·Wolfgang M ProdingerIrmgard Moser
May 17, 2014·Research in Veterinary Science·E GormleyS Rodriguez-Campos
Dec 24, 2013·Research in Veterinary Science·Laura Pérez-LagoDarío García-de-Viedma
Sep 2, 2009·Veterinary Microbiology·Sabrina RodríguezUNKNOWN Spanish Network on Surveillance and Monitoring of Animal Tuberculosis
May 7, 2005·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·M S ZaniniM J Zumarraga
Aug 17, 2005·Emerging Infectious Diseases·Andrea GoriFabio Franzetti

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