Restriction fragment length polymorphism of rRNA genes for molecular typing of members of the family Legionellaceae.

Journal of Clinical Microbiology
J M BangsborgN Høiby

Abstract

Typing of Legionella pneumophila remains important in the investigation of outbreaks of Legionnaires' disease and in the control of organisms contaminating hospital water. We found that the discriminatory power of a nonradioactive ribotyping method could be improved by combining results obtained with four restriction enzymes (HindIII, NciI, ClaI, and PstI). Fifty-eight clinical and environmental L. pneumophila strains including geographically unrelated as well as epidemiologically connected isolates were investigated. Epidemiologically related strains had the same ribotypes independent of the combinations of enzymes used. Some strains belonging to the same serogroup were assigned to different ribotypes, and some ribotypes contained members of different serogroups, indicating, as others have found, that serogroup and genotype are not always related. The discriminatory power of the method was estimated by calculating an index of discrimination (ID) for individual enzymes and combinations thereof. The combined result with all four enzymes was highly discriminatory (ID = 0.97), but results for three enzymes also yielded ID values acceptable for epidemiological purposes. In addition, the testing of 27 type strains and 6 clinical iso...Continue Reading

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Citations

Mar 1, 1997·Avian Pathology : Journal of the W.V.P.A·J K MiflinP J Blackall
Jan 9, 2003·Journal of Clinical Microbiology·Christophe CordevantMarc Lange
Jun 1, 1996·Journal of Clinical Microbiology·F GrattardB Pozzetto
Mar 15, 2003·International Journal of Medical Microbiology : IJMM·Vladislava RůzickováStanislav Rosypal

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