PMID: 2496380Mar 1, 1989Paper

Restriction fragment mapping of nontypable haemophilus influenzae: a new tool to study this middle ear pathogen

Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery
J M BernsteinD P Dickinson

Abstract

Isolates of nontypable haemophilus influenzae (NTHI) recovered from paired nasopharyngeal (NP) and middle ear (ME) fluid cultures performed simultaneously on seven children with otitis media with effusion (OME) were studied by analysis of the restriction fragment patterns produced from total genomic DNA. This method provides a sensitive measure of the genetic similarities between strains. In the seven pairs examined, the NP and ME strains were indistinguishable from one another, whereas each pair was distinct from the other, except for two siblings in whom all four strains were identical. This information provides evidence that the pathogenesis of OME caused by NTHI involves spread of the bacteria from NP to the ME. Analysis of paired NP and ME isolates from three children with recurrent OME caused by NTHI indicated that the second episode was caused by the reinfection with a different strain rather than persistence and reemergence of the first strain. Finally, DNA analysis of strains from two siblings with concurrent OME suggested that person-to-person transmission of NTHI can occur among children. These findings suggest that at any one time the NTHI population in the NP is relatively homogenous, at least at the time of acute ...Continue Reading

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Citations

Jul 1, 1993·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·C HemlinA Scheynius
Jul 1, 1992·International Journal of Pediatric Otorhinolaryngology·D WangM P Derde
Nov 1, 1990·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·J M BernsteinP L Ogra
Feb 1, 1994·The Journal of Laryngology and Otology·J Sadé
Dec 8, 2004·ORL; Journal for Oto-rhino-laryngology and Its Related Specialties·Muneki HotomiKinoyori Kuki
Jan 24, 1998·The Annals of Otology, Rhinology, and Laryngology·J M BernsteinK O'Neil
May 20, 2009·Acta Oto-laryngologica·Anna GranathLars-Olaf Cardell
Mar 1, 1991·The Annals of Otology, Rhinology, and Laryngology·J Sadé, M Luntz

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