Restriction-modification systems and bacteriophage invasion: who wins?

Journal of Theoretical Biology
Farida N EnikeevaMikhail S Gelfand

Abstract

The success of a phage that infects a bacterial cell possessing a restriction-modification (R-M) system depends on the activities of the host methyltransferase and restriction endonuclease, and the number of susceptible sites in the phage genome. However, there is no model describing this dependency and linking it to observable parameters such as the fraction of surviving cells under excess phage, or probability of plating at low amount of phages. We model the phage infection of a cell with a R-M system as a pure birth process with a killing state. We calculate the transitional probabilities and the stationary distribution for this process. We generalize the model developed for a single cell to the case of multiple identical cells invaded by a Poisson-distributed number of phages. The R-M enzyme activities are assumed to be constant, time-dependent, or random. The obtained results are used to estimate the ratio of the methyltransferase and endonuclease activities from the observed fraction of surviving cells.

References

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Citations

Jul 4, 2013·Nature Communications·Marie-Ève DupuisSylvain Moineau
Oct 15, 2013·Applied and Environmental Microbiology·James MurphyDouwe van Sinderen
Jan 26, 2016·Current Biology : CB·Maroš PleškaCălin C Guet
Jun 6, 2012·Bacteriophage·Stephen T Abedon
Jul 3, 2019·PLoS Computational Biology·Jakob RuessGašper Tkačik
Feb 11, 2015·Acta Crystallographica. Section D, Biological Crystallography·M B ShevtsovG G Kneale
May 8, 2021·Frontiers in Microbiology·Kamil SteczkiewiczAgnieszka K Szczepankowska
Apr 6, 2021·Critical Reviews in Microbiology·Avijit GoswamiRachit Agarwal
Sep 15, 2021·Nucleic Acids Research·Fredj Ben BdiraRemus T Dame

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