Restriction of Epstein-Barr virus-specific cytotoxic T cells by HLA-A, -B, and -C molecules.

Human Immunology
B P ChenP M Sondel

Abstract

HLA-loss variants of an Epstein-Barr virus-transformed B-lymphoblastoid cell line (EBV-LCL) 721 were used as target cells to identify HLA molecules utilized by EBV-LCL-specific cytotoxic T cells. Split culture analysis of cytotoxic T cells plated at limiting dilution showed killing of HLA-loss variants bearing either HLA-A2 or -B5 molecules, with 10 times higher frequency of cytotoxic T cells restricted by the HLA-B5 molecule. Clonal analysis confirmed the restriction by HLA-A2 or -B5 of some cytotoxic T-cell clones and identified cytotoxic T-cell clones cytolytic for target cells which do not express HLA-A or -B but do express the HLA-C determinant. Thus, our results show immunodominance of the HLA-B5 restriction determinant for EBV-induced antigens in the donor of the HLA-loss variants and provide evidence that the HLA-C molecule can also serve as restriction determinant for EBV-LCL-specific cytotoxic T cells.

References

Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·O DillG J Hämmerling
Feb 1, 1987·The Journal of Experimental Medicine·F GotchB Moss
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D A Thorley-Lawson, E S Israelsohn
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·Y ShimizuR DeMars
Nov 3, 1988·Nature·A M NormentD R Littman
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R DeMarsH T Orr
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P KavathasR DeMars
Nov 1, 1985·Immunology Today·R B Mandell, P M Sondel

❮ Previous
Next ❯

Citations

Jul 1, 1995·Medical Hypotheses·L KomlosI Halbrecht
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K FalkH G Rammensee
Oct 1, 1992·The Journal of Experimental Medicine·J Zemmour, P Parham
Feb 24, 2015·The Journal of Allergy and Clinical Immunology·Ömür ArdenizKlaus Warnatz
Mar 1, 1997·Leukemia & Lymphoma·K G LucasD J Emanuel
Sep 1, 1994·European Journal of Immunology·P van der BruggenT Boon
Jan 1, 1997·Immunologic Research·C S Falk, D J Schendel

❮ Previous
Next ❯

Related Concepts

Related Feeds