Abstract
Renal-cell carcinoma (RCC) is one of the human cancers which respond best to immunotherapy. To better characterize the mechanism of the immune response in RCC, we analyzed the T-cell receptor (TCR) beta-chain repertoire in primary RCC, metastases and paired peripheral blood lymphocytes (PBL) from 9 patients. For 3 of these, we analyzed T cells recovered from normal kidney, or from tumor-involved lymph nodes as well as tumor-infiltrating lymphocytes (TIL) expanded in vitro for adoptive immunotherapy. The initial semi-quantitative RT-PCR method for definition of the Vbeta gene usage was not informative enough to distinguish intratumoral clonal T-cell expansions. In contrast, the length pattern analysis of the complementary determining regions 3 (CDR3) allowed oligoclonal T-cell populations to be detected in fresh TIL form the 9 patients with RCC. Furthermore, these oligoclonal TIL populations were not present in normal renal tissue, autologous PBL or tumor-involved lymph nodes. Different clonal T-cell expansions were identified in the primary tumor and in a pulmonary metastasis from the same patient. The detection of clonal T-cell populations observed in RCC suggests an in situ expansion in response to potential tumor antigens. T...Continue Reading
References
Oct 1, 1992·European Journal of Immunology·M CochetP Kourilsky
May 1, 1992·European Journal of Immunology·C GenevéeT Hercend
May 1, 1992·British Journal of Cancer·S NégrierM Clavel
Jan 1, 1992·Journal of Immunotherapy·J H FinkeR Bukowski
May 15, 1986·Nature·P J FinkS M Hedrick
Aug 4, 1988·Nature·M M Davis, P J Bjorkman
Mar 15, 1987·International Journal of Cancer. Journal International Du Cancer·M HérinT Boon
Jul 1, 1995·Immunology Today·T BoonP G Coulie
Feb 1, 1995·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Y MerroucheT Philip
Jan 1, 1994·Immunogenetics·S WeiP Concannon
Mar 1, 1993·The Journal of Clinical Investigation·L FerradiniT Hercend
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C PannetierP Kourilsky
Jan 1, 1987·Immunology Today·A AnichiniG Parmiani
Citations
Jul 16, 2005·Hematology·Yangqiu LiSuxia Geng
Sep 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Oumeya AdjaliNaomi Taylor
Aug 3, 2005·The Journal of Clinical Investigation·Oumeya AdjaliDavid Klatzmann
Jun 2, 2000·Human Gene Therapy·C FerrandP Tiberghien
May 8, 1999·British Journal of Haematology·T ShimomuraM Kawakita
Sep 29, 2012·Immunological Investigations·Peter A CohenJames H Finke
Oct 13, 2004·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Min HuStephen I Alexander
Oct 15, 2013·The Journal of Pathology·Marco GerlingerCharles Swanton
Dec 12, 2003·Hematology·Yangqiu Li
Jan 3, 2006·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Géraldine GallotHenri Vié
Jul 29, 1997·International Journal of Cancer. Journal International Du Cancer·E AngevinF Triebel
Sep 17, 1996·International Journal of Cancer. Journal International Du Cancer·C BainM Favrot
Oct 6, 1997·Journal of the National Cancer Institute·Y MerroucheM Favrot
Sep 29, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kenzaburo TaniShigetaka Asano
Mar 9, 1999·International Immunology·D N PosnettG Marodon
Feb 10, 1999·Medical Oncology·E Halapi
Mar 10, 2009·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Christiane GeigerDolores J Schendel
Jun 30, 2004·Radiation Research·William H McBrideYu-Pei Liao
Aug 1, 2002·Blood·Makoto OtsuNelly Noraz
Sep 5, 2001·Clinical and Experimental Immunology·D J KingUNKNOWN Paediatric European Network for Treatment of AIDS (PENTA)
Sep 10, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jayanthi ManneDavid Berd
Jul 2, 1999·Springer Seminars in Immunopathology·E HalapiH Mellstedt
Apr 28, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Andreas E AlbersAlbert B De Leo
Sep 28, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Patricia RaymanJames Finke