Results with an apparatus for pressurized planar electrochromatography

Analytical Chemistry
Allyson L NovotnyRobert E Santini

Abstract

Pressurized planar electrochromatography (PPEC) is a fast and efficient planar chromatographic technique. The mobile phase is driven by electroosmotic flow, while the system is pressurized in a manner that allows heat to flow between the sorbent layer and the pressurizing medium. The reproducibility of solute retention was not satisfactory in the initial report describing PPEC. In the current report, this reproducibility is improved by better control of several experimental variables. The pressure at which PPEC is performed is now free of drift, and the temperature at which the layer is preconditioned is maintained to within +/-1 degrees C. The best reproducibility of retention is obtained when the plate is soaked in the mobile phase for a defined time before each run. In the original prototype, the temperature of the sorbent layer was not controlled. In the present apparatus, water, at a constant temperature between 3 and 60 degrees C, is circulated through channels in the two die blocks that pressurize the layer. The highest efficiency is obtained at an intermediate temperature. This behavior is ascribed to high resistance to mass transfer at the lower temperatures and increased diffusion at higher temperatures. Efficiency, a...Continue Reading

References

Feb 6, 2003·Journal of Chromatography. a·David NurokMatthew A Carmichael
Mar 17, 2004·Analytical Chemistry·David NurokRandall W Replogle
Sep 10, 2004·Journal of Chromatography. a·David Nurok

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Citations

Apr 9, 2008·Analytical and Bioanalytical Chemistry·Tadeusz H DzidoAneta Hałka
Jan 30, 2013·Analytical and Bioanalytical Chemistry·Peter R StevensonDavid C Collins
Feb 28, 2014·Journal of Chromatography. a·Tadeusz H DzidoHartmut Frank
Jul 9, 2009·Journal of Separation Science·Pingli WangYukui Zhang
Feb 9, 2019·Journal of Analytical Methods in Chemistry·Beata PolakMałgorzata Kozyra
Oct 23, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Sergio MendezGabriel P López
Jan 9, 2010·Analytical Chemistry·Joseph Sherma
Oct 22, 2014·Analytical Chemistry·Toni E Newsome, Susan V Olesik

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