Resurrection of the flagellar rotary motor near zero load.

Proceedings of the National Academy of Sciences of the United States of America
Junhua Yuan, Howard C Berg

Abstract

Flagellated bacteria, such as Escherichia coli, are propelled by helical flagellar filaments, each driven at its base by a reversible rotary motor, powered by a transmembrane proton flux. Torque is generated by the interaction of stator proteins, MotA and MotB, with a rotor protein FliG. The physiology of the motor has been studied extensively in the regime of relatively high load and low speed, where it appears to operate close to thermodynamic equilibrium. Here, we describe an assay that allows systematic study of the motor near zero load, where proton translocation and movement of mechanical components are rate limiting. Sixty-nanometer-diameter gold spheres were attached to hooks of cells lacking flagellar filaments, and light scattered from a sphere was monitored at the image plane of a microscope through a small pinhole. Paralyzed motors of cells carrying a motA point mutation were resurrected at 23 degrees C by expression of wild-type MotA, and speeds jumped from zero to a maximum value ( approximately 300 Hz) in one step. Thus, near zero load, the speed of the motor is independent of the number of torque-generating units. Evidently, the units act independently (they do not interfere with one another), and there are no i...Continue Reading

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Citations

Sep 25, 2008·Quarterly Reviews of Biophysics·Yoshiyuki Sowa, Richard M Berry
Apr 14, 2012·Nature·Junhua YuanHoward C Berg
Feb 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·Giovanni Meacci, Yuhai Tu
Jul 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Junhua YuanHoward C Berg
Sep 30, 2010·Proceedings of the National Academy of Sciences of the United States of America·Shuichi NakamuraKeiichi Namba
Feb 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Yilin WuHoward C Berg
Dec 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Michael W SneddonThierry Emonet
Nov 22, 2012·Proceedings of the National Academy of Sciences of the United States of America·Pushkar P LeleHoward C Berg
Jun 22, 2013·Proceedings of the National Academy of Sciences of the United States of America·Chien-Jung LoRichard M Berry
Jun 4, 2013·Applied and Environmental Microbiology·Chen QianKeng-Hwee Chiam
Mar 27, 2012·Journal of Bacteriology·Linda TurnerHoward C Berg
Aug 22, 2013·MBio·Murray J TippingJudith P Armitage
Oct 24, 2009·PLoS Computational Biology·Thierry MoraNed S Wingreen
Jul 31, 2012·PloS One·Henry G ZotNguyen Van Minh
Jul 3, 2013·Proceedings of the National Academy of Sciences of the United States of America·Pushkar P LeleHoward C Berg
Dec 8, 2010·The Review of Scientific Instruments·Yoshiyuki SowaRichard M Berry
Jun 28, 2014·Biomolecules·Yusuke V Morimoto, Tohru Minamino
Oct 22, 2014·Proceedings of the National Academy of Sciences of the United States of America·Fangbin WangHoward C Berg
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Sep 16, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Andreas Diepold, Judith P Armitage
Apr 14, 2016·Proceedings of the National Academy of Sciences of the United States of America·Basarab G HosuHoward C Berg
Jul 29, 2015·Proceedings of the National Academy of Sciences of the United States of America·Kranthi K MandadapuGeorge Oster
Jul 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Teuta PilizotaJudith P Armitage
Jan 10, 2009·Journal of Molecular Biology·Shuichi NakamuraKeiichi Namba
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May 21, 2010·Biophysical Journal·Junhua Yuan, Howard C Berg
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Nov 8, 2017·Proceedings of the National Academy of Sciences of the United States of America·Bin WangJunhua Yuan
Jul 25, 2019·Biomolecules·Shuichi Nakamura, Tohru Minamino
Dec 1, 2017·Proceedings of the National Academy of Sciences of the United States of America·Ashley L NordFrancesco Pedaci
Mar 25, 2019·Science Advances·Hui ShiJunhua Yuan

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