PMID: 15229363Jul 2, 2004Paper

Resuscitation affects microcirculatory polymorphonuclear leukocyte behavior after hemorrhagic shock: role of hypertonic saline and pentoxifylline

Experimental Biology and Medicine
M M Yada-LanguiRaul Coimbra

Abstract

We have previously shown that lung injury following fluid resuscitation either with hypertonic saline (HS) or lactated Ringer's (LR) plus pentoxifylline (PTX) attenuated acute lung injury when compared with LR resuscitation. The objective of the present study is to determine whether our previous observations are accompanied by changes in polymorphonu-clear leukocyte (PMN) behavior. To study this, PMN-endothelial cell interactions, microcirculatory blood flow, lung histology, lung PMN infiltration (MPO, Myeloperoxidase), and lung intra-cellular adhesion molecule-1 (ICAM-1) expression were assessed in a controlled hemorrhagic shock model followed by LR, HS, and LR+PTX resuscitation in rodents. Rats (240-300 g) were bled to a mean arterial pressure (MAP) of 35 mm Hg for 1 hr and then randomized into three groups: HS (7.5% NaCl, 4 ml/kg); LR (3x shed blood); and LR+PTX (25 mg/kg). Additionally, total shed blood was reinfused. A sham group underwent no shock and no treatment. The internal spermatic fascia was exteriorized and the microcirculation was observed by closed-circuit TV coupled to a microscope, 2 and 6 hrs after treatment. The number of leukocytes sticking to the venular endothelium was determined 2 hrs after fluid resusci...Continue Reading

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Citations

Jul 3, 2008·Plastic and Reconstructive Surgery·Thomas Scholz, Gregory R D Evans
Sep 30, 2009·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·C I FernandesH P Souza
Jul 21, 2020·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Anoek L I van LeeuwenCharissa E van den Brom
Jun 2, 2005·Current Opinion in Critical Care·Colin Verdant, Daniel De Backer
Aug 24, 2011·The Journal of Trauma·Raúl Coimbra
Feb 19, 2008·Shock·Miriam S Giambelluca, Oscar A Gende
Apr 19, 2007·Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde·Turgut YilmazBilal Ustundağ
Aug 3, 2010·Journal of Vascular Surgery·Nilon ErlingLuiz Francisco Poli-de-Figueiredo

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