Resveratrol alleviates hypoxia/reoxygenation injury‑induced mitochondrial oxidative stress in cardiomyocytes

Molecular Medicine Reports
Tao LiXiao-Qiu Tan

Abstract

Resveratrol (RES) is a naturally occurring antioxidant compound found in red wine. Although it has been demonstrated to have a cardioprotective effect, the mechanism underlying this effect remains to be fully elucidated. The aim of the present study was to determine whether RES exerts a protective effect against mitochondrial oxidative stress and apoptosis in neonatal rat cardiomyocytes (NRCMs) induced by hypoxia/reoxygenation (H/R) injury. Primary cultured NRCMs were used as a model system and were divided into four experimental groups: Control, H/R, H/R + DMSO (H/R with 0.2% DMSO) and H/R + RES (H/R with 100 µM RES) groups. Mitochondrial oxidative stress was determined by measuring the alteration in the mitochondrial membrane potential (ΔΨm) of NRCMs, the release of lactate dehydrogenase (LDH) and the ratio of B‑cell lymphoma 2 (Bcl‑2)/Bcl‑2‑associated X protein (Bax) from NRCMs. Cell apoptosis was assessed by measuring cell apoptotic rates and the activity of caspase 3. In the H/R+RES group, RES significantly alleviated structural impairment, including disordered α‑actin and F‑actin, in the NRCMs induced by H/R injury. RES attenuated H/R injury‑induced mitochondria oxidative stress. RES also attenuated H/R injury‑induced cel...Continue Reading

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Citations

Aug 21, 2019·International Journal of Molecular Sciences·Afonso Caricati-NetoFrancisco Sandro Menezes-Rodrigues
Feb 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Michał OtrębaAnna Rzepecka-Stojko
Jun 3, 2021·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Yupeng LiuJingchun He
Jul 10, 2021·Oxidative Medicine and Cellular Longevity·Judith Bernal-RamírezGerardo García-Rivas

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Methods Mentioned

BETA
Assay
protein assay
flow cytometry

Software Mentioned

SPSS

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