Resveratrol Alleviates Inflammatory Responses and Oxidative Stress in Rat Kidney Ischemia-Reperfusion Injury and H2O2-Induced NRK-52E Cells via the Nrf2/TLR4/NF-κB Pathway

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Jiawei LiRuiming Rong

Abstract

Ischemia-reperfusion injury (IRI) is one of the major causes of postoperative renal allograft dysfunction, which is mainly the result of proinflammatory reactions including inflammatory responses, oxidative stress, and metabolic disorders. Resveratrol (RSV) plays an important role in protecting various organs in IRI because it reduces oxidative stress, lessens the inflammatory response, and exerts anti-apoptotic effects. The aim of this study was to demonstrate the renoprotective effect of RSV in inhibiting inflammatory responses, reducing oxidative stress, and decreasing cell apoptosis in vivo and in vitro. RSV was administered before renal ischemia and H2O2 induction. Serum and kidneys were harvested 24 h after reperfusion and NRK-52E cells were collected 4 h after H2O2 stimulation. Serum creatinine and blood urea nitrogen were used to assess renal function. Hematoxylin and eosin staining was performed to assess histological injury. Quantitative real-time PCR and enzyme-linked immunosorbent assay were used to assess proinflammatory cytokine expression. Oxidative stress-related proteins, such as Nrf2 and TLR4, were evaluated by western blot. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay was...Continue Reading

Citations

Aug 18, 2018·Clinical and Experimental Pharmacology & Physiology·Zhaohui LiuYulin Chang
Jun 20, 2018·International Urology and Nephrology·Isabelle C V S MartinsDenise Mafra
Apr 11, 2019·Current Pharmaceutical Design·Tanzir RafeMd Abu Taher Sagor
Apr 26, 2020·International Journal of Molecular Sciences·Masahiro Nezu, Norio Suzuki
Oct 20, 2018·Frontiers in Neuroscience·Luyao XuXuehong Liu
Mar 9, 2019·International Journal of Molecular Sciences·Vanesa IzquierdoChristian Griñán-Ferré
Dec 1, 2020·Journal of Biochemical and Molecular Toxicology·Jing WangLingling Shan
Jan 6, 2021·Antioxidants·Melania Guerrero-HueJuan Antonio Moreno
Feb 6, 2021·Nutrition Research and Practice·Benxi XiaoDuan Wang
Feb 27, 2020·American Journal of Surgery·Gabriela F Buys-GonçalvesDiogo B De Souza
Jul 11, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Fevzi BedirHalis Suleyman
Aug 8, 2021·International Journal of Molecular Sciences·Md Jamal UddinHunjoo Ha
Jun 11, 2021·Journal of the American Society of Nephrology : JASN·Shelby Reid, James W Scholey
Aug 25, 2021·Transplantation Proceedings·Xu ZhenLiang Sudong

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Caspases in Metabolic Diseases

Caspases, the family of cysteine proteases are involved in programmed cell death, but their role in metabolic diseases, inflammation and immunity has been of interested. Discover the latest research on caspases in metabolic diseases here.