Resveratrol ameliorates the maturation process of β-cell-like cells obtained from an optimized differentiation protocol of human embryonic stem cells

PloS One
Daniela PezzollaBernat Soria

Abstract

Human embryonic stem cells (hESCs) retain the extraordinary capacity to differentiate into different cell types of an adult organism, including pancreatic β-cells. For this particular lineage, although a lot of effort has been made in the last ten years to achieve an efficient and reproducible differentiation protocol, it was not until recently that this aim was roughly accomplished. Besides, several studies evidenced the impact of resveratrol (RSV) on insulin secretion, even though the mechanism by which this polyphenol potentiates glucose-stimulated insulin secretion (GSIS) is still not clear. The aim of this study was to optimize an efficient differentiation protocol that mimics in vivo pancreatic organogenesis and to investigate whether RSV may improve the final maturation step to obtain functional insulin-secreting cells. Our results indicate that treatment of hESCs (HS-181) with activin-A induced definitive endoderm differentiation as detected by the expression of SOX17 and FOXA2. Addition of retinoic acid (RA), Noggin and Cyclopamine promoted pancreatic differentiation as indicated by the expression of the early pancreatic progenitor markers ISL1, NGN3 and PDX1. Moreover, during maturation in suspension culture, differen...Continue Reading

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Citations

Jul 15, 2015·Expert Opinion on Biological Therapy·Bernat SoriaAbdelkrim Hmadcha
Dec 27, 2015·Nutrition·Gustavo Tomas Diaz-GereviniAldo Renato Eynard
Aug 1, 2018·Current Drug Targets·E Paul CherniackHeather F Lee
May 27, 2016·Cardiovascular Therapeutics·Hong DingQiuting Feng
Sep 19, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Na LiXiaohua Li
Apr 3, 2020·International Journal of Molecular Sciences·Gitika ThakurGyu-Jin Rho
Jun 22, 2017·Frontiers in Genetics·Andhira VieiraPatrick Collombat
Dec 19, 2020·Open Medicine·Michael D WilliamsWilson K M Wong
Jul 3, 2021·In Vitro Cellular & Developmental Biology. Animal·Mehrdad VakilianKamran Ghaedi

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Methods Mentioned

BETA
ELISA
immunoprecipitation
ChIP
fluorescence imaging
PCR
electrophoresis

Software Mentioned

LabScan
ImmageQuant
MetaMorph

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