Resveratrol and Pterostilbene Exhibit Anticancer Properties Involving the Downregulation of HPV Oncoprotein E6 in Cervical Cancer Cells

Nutrients
Kaushiki ChatterjeeJimmie E Fata

Abstract

Cervical cancer is one of the most common cancers in women living in developing countries. Due to a lack of affordable effective therapy, research into alternative anticancer compounds with low toxicity such as dietary polyphenols has continued. Our aim is to determine whether two structurally similar plant polyphenols, resveratrol and pterostilbene, exhibit anticancer and anti-HPV (Human papillomavirus) activity against cervical cancer cells. To determine anticancer activity, extensive in vitro analyses were performed. Anti-HPV activity, through measuring E6 protein levels, subsequent downstream p53 effects, and caspase-3 activation, were studied to understand a possible mechanism of action. Both polyphenols are effective agents in targeting cervical cancer cells, having low IC50 values in the µM range. They decrease clonogenic survival, reduce cell migration, arrest cells at the S-phase, and reduce the number of mitotic cells. These findings were significant, with pterostilbene often being more effective than resveratrol. Resveratrol and to a greater extent pterostilbene downregulates the HPV oncoprotein E6, induces caspase-3 activation, and upregulates p53 protein levels. Results point to a mechanism that may involve the dow...Continue Reading

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Citations

Jan 16, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hee Jeong ShinHye Jin Jung
Apr 11, 2019·Current Pharmaceutical Design·Tanzir RafeMd Abu Taher Sagor
Feb 27, 2021·Frontiers in Cell and Developmental Biology·Natália Lourenço de FreitasChristiane P Soares
Feb 21, 2019·Journal of Agricultural and Food Chemistry·Dana M PoloniJoe A Vinson
Sep 11, 2021·Molecular Nutrition & Food Research·Yen-Chun KohMin-Hsiung Pan

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Methods Mentioned

BETA
electrophoresis

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GraphPad Prism
Excel
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