Resveratrol attenuates brain damage in permanent focal cerebral ischemia via activation of PI3K/Akt signaling pathway in rats

Neurological Research
Junrong Lei, Qianxue Chen

Abstract

The aim of this study was to evaluate the potential molecular mechanism of resveratrol (RSV) that attenuates brain damage from focal cerebral ischemia. To investigate whether phosphatidylinositol 3-kinase/Akt (PI3K/Akt) pathway was involved in RSV anti-inflammatory and neuroprotective properties. Middle cerebral artery occlusion (MCAO) animal model was used in this study. Adult male Sprague-Dawley (SD) rats underwent MCAO, and then received treatment with RSV or vehicle after the onset of ischemia. PI3K inhibitor LY294002 was injected intracerebroventricularly to inhibit the PI3K/Akt signaling pathway. Neurological deficit scores and cerebral water content were assessed 24 h after MCAO. The inflammatory factors interleukin (IL)-1β, tumor necrosis factor (TNFα), and cyclooxygenase-2 (COX2) mRNA level were examined by real-time PCR. The enzymatic activity of myeloperoxidase (MPO) was measured 24 h after MCAO. The protein expression of phospho-Akt and COX2 in ischemic brain were determined by western blot. RSV significantly reduced neurological deficit scores, cerebral water content and the enzymatic activity of MPO, all of which were abolished by LY294002 administration. Real-time PCR showed that RSV significantly suppressed the ...Continue Reading

References

May 1, 1986·Stroke; a Journal of Cerebral Circulation·J B BedersonH Bartkowski
Aug 11, 1999·Trends in Neurosciences·U DirnaglM A Moskowitz
May 30, 2006·Nature Reviews. Drug Discovery·Joseph A Baur, David A Sinclair
Aug 23, 2006·Molecular and Cellular Biochemistry·Ozkan AtesSaim Yologlu
Oct 25, 2006·Archives of Internal Medicine·Mitchell S V ElkindRalph L Sacco
Apr 15, 2008·Neurochemical Research·Martin B Gill, J Regino Perez-Polo
Feb 5, 2010·Journal of Leukocyte Biology·Rong JinGuohong Li
Jun 15, 2010·Free Radical Biology & Medicine·Masayuki FukuiBao Ting Zhu
Oct 20, 2010·Annals of the New York Academy of Sciences·Gregory J del Zoppo
Jan 10, 2013·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Jinying ZhangGuangtao Yan
Jan 29, 2013·The Journal of Trauma and Acute Care Surgery·Joshua W GatsonJoseph P Minei
Oct 19, 2013·Stroke; a Journal of Cerebral Circulation·María Isabel CuarteroMaría A Moro
Nov 1, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Arthur LieszRoland Veltkamp
Feb 25, 2014·Pharmacology, Biochemistry, and Behavior·Dhirendra Pratap Singh, Kanwaljit Chopra
Oct 14, 2014·Frontiers in Aging Neuroscience·Shraddha D RegeJeganathan Ramesh Babu

❮ Previous
Next ❯

Citations

Jun 27, 2020·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Meng ChaoYaping Huang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.