Resveratrol attenuates renal injury and fibrosis by inhibiting transforming growth factor-β pathway on matrix metalloproteinase 7

Experimental Biology and Medicine
Zhou XiaoQiaoling Zhou

Abstract

Renal injury has a strong relationship to the subsequent development of renal fibrosis. In developing renal fibrosis, tubular epithelial cells in the kidney underwent epithelial-mesenchymal transition (EMT). Matrix metalloproteinase 7 (MMP7) was reported to reduce E-cadherin and induce EMT by up-regulation of β-catenin/lymphoid enhancer-binding factor 1 (LEF1) signaling. In this research, we tried to evaluate the role of resveratrol (RSV) on EMT process in renal injury and fibrosis. Human tubular epithelial cell HK-2 cells were treated with aristolochic acid (AAs) and transforming growth factor-β(TGF-β) to induce EMT with or without the administration of RSV. The inhibitory role of RSV on EMT in renal injury and fibrosis was determined by Western blotting, real-time PCR, and immunofluorescence staining. The EMT repressing role of RSV was also evaluated in vivo by renal ischemia-reperfusion (I/R) injury and unilateral ureteral obstruction (UUO) models. The underlying mechanism was investigated by shRNA interfering MMP7 and sirtuin 1 (SIRT1) expression. The results indicated that RSV reversed human kidney 2 (HK-2) cell EMT, renal I/R injury, and renal fibrosis. MMP7 inhibition was responsible for RSV-induced EMT repression. SIRT1...Continue Reading

References

Feb 19, 2000·Life Sciences·L Frémont
May 22, 2003·The American Journal of Pathology·John K McGuireWilliam C Parks
Aug 27, 2003·Nature·Konrad T HowitzDavid A Sinclair
Aug 27, 2005·Oncogene·Jiri Zavadil, Erwin P Böttinger
Dec 27, 2005·Journal of Vascular Surgery·Vikas Chander, Kanwaljit Chopra
Feb 21, 2006·The Journal of Surgical Research·Meltem KolgaziInci Alican
Nov 10, 2006·The EMBO Journal·Agnès BoutetM Angela Nieto
Aug 19, 2007·Life Sciences·Telma de Jesus SoaresTerezila M Coimbra
Nov 13, 2008·Laboratory Investigation; a Journal of Technical Methods and Pathology·Takanori KitamuraMakoto M Taketo
Dec 1, 2009·Cell·Jean Paul ThieryM Angela Nieto
Mar 26, 2010·The Journal of Clinical Investigation·Wenjuan HeChuan-Ming Hao
Jun 10, 2011·The New England Journal of Medicine·Leonard Guarente
Jan 1, 2014·Oxidative Medicine and Cellular Longevity·Munehiro Kitada, Daisuke Koya
Jun 19, 2014·Experimental Biology and Medicine·Chengxing LiuWeiyi Chen

❮ Previous
Next ❯

Citations

Jul 6, 2016·Oxidative Medicine and Cellular Longevity·Raffaele PalmirottaFranco Silvestris
Jul 28, 2016·International Journal of Molecular Sciences·Akinori Tokito, Michihisa Jougasaki
Oct 19, 2017·Food & Function·Abdur RaufMohammad S Mubarak
Jun 20, 2019·International Journal of Molecular Sciences·Jong Han LeeHee-Sook Jun
Nov 16, 2019·Minerva urologica e nefrologica = The Italian journal of urology and nephrology·Raquel EchavarríaZesergio Melo
Apr 28, 2020·Expert Opinion on Therapeutic Targets·Sandra Rayego-Mateos, Jose M Valdivielso
Aug 25, 2020·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·Ayşegül Kapucu
Feb 28, 2017·Frontiers in Physiology·Ben KeXiangdong Fang
Feb 15, 2019·Frontiers in Pharmacology·Vincenzo CarafaAngela Nebbioso
Jul 20, 2019·Nutrients·Danja J Den Hartogh, Evangelia Tsiani
Aug 17, 2019·Frontiers in Pharmacology·Lorena Avila-CarrascoGuadalupe González Mateo
Sep 17, 2020·International Journal of Molecular Sciences·Yu Ah HongGang-Jee Ko
Jul 8, 2020·Biomolecules·Zhao LiuYouhua Liu
Feb 27, 2020·American Journal of Surgery·Gabriela F Buys-GonçalvesDiogo B De Souza
May 1, 2021·International Journal of Molecular Sciences·Chien-Ning HsuYou-Lin Tain
Aug 8, 2021·International Journal of Molecular Sciences·Md Jamal UddinHunjoo Ha
Aug 7, 2021·Advanced Drug Delivery Reviews·Huan XuLeaf Huang
Aug 27, 2021·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Inger Therese T EnoksenToralf Melsom

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
ELISA
immunoprecipitations
acetylation
transfection

Related Concepts

Related Feeds

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.