Resveratrol delays replicative senescence of human mesothelial cells via mobilization of antioxidative and DNA repair mechanisms

Free Radical Biology & Medicine
Justyna Mikuła-PietrasikKrzysztof Ksiazek

Abstract

Resveratrol (3,4',5-trihydroxy-trans-stilbene; RVT) is a natural phytoestrogen known to modulate the rate of senescence in cultured cells. The mechanism by which RVT affects this process is still elusive. In this paper we used human peritoneal mesothelial cells (HPMCs) to examine the effect of RVT (0.5 and 10 μM) on their growth and senescence, with particular emphasis paid to parameters associated with oxidative stress. The results showed that RVT used at a concentration of 0.5 μM (but not at 10 μM) markedly improved HPMC growth capacity, as evidenced by elevated expression of PCNA antigen, augmented fraction of cells in the S phase of the cell cycle, and increased number of divisions achieved before senescence. These effects coincided with diminished expression and activity of senescence-associated β-galactosidase but were not associated with changes in the telomere length and an incidence of apoptosis. Moreover cells exposed to 0.5 μM RVT were characterized by increased release of reactive oxygen species, which was accompanied by up-regulated biogenesis of mitochondria and collapsed mitochondrial membrane potential. At the same time, they displayed increased activity of superoxide dismutase and reduced DNA damage (8-OH-dG an...Continue Reading

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Citations

Aug 29, 2012·Oxidative Medicine and Cellular Longevity·Samo Ribarič
Jul 11, 2013·Oxidative Medicine and Cellular Longevity·Khushwant S Bhullar, H P Vasantha Rupasinghe
Oct 5, 2015·Clinical & Experimental Metastasis·Justyna Mikuła-PietrasikKrzysztof Książek
Oct 3, 2015·Nutrition·Michael J Glade, Michael M Meguid
Sep 23, 2014·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Justyna Mikuła-PietrasikKrzysztof Książek
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Dec 30, 2016·Cell Death & Disease·Justyna Mikuła-PietrasikKrzysztof Książek
Jun 28, 2017·Free Radical Biology & Medicine·Justyna Mikuła-PietrasikKrzysztof Książek
Jul 1, 2020·International Journal of Molecular Sciences·Ekaterina ProshkinaAlexey Moskalev
Aug 19, 2015·Oncotarget·Justyna Mikuła-PietrasikKrzysztof Książek
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