Resveratrol Downregulates miR-31 to Promote T Regulatory Cells during Prevention of TNBS-Induced Colitis

Molecular Nutrition & Food Research
Haider Rasheed AlrafasPrakash S Nagarkatti

Abstract

Colitis, an inflammatory bowel disease, is associated with aberrant regulation of the colonic mucosal immune system. Resveratrol, a natural plant product, has been found to exert anti-inflammatory properties and attenuate the development of murine colitis. In the current study, the role of microRNA (miR) in the ability of resveratrol to suppress colonic inflammation is examined. BALB/C mice with 2,4,6-Trinitrobenzenesulfonic acid solution (TNBS)-induced colitis, when treated with resveratrol, show improved clinical outcomes and reduce induction of inflammatory T cells (Th17 and Th1) while increasing CD4+Foxp3+ regulatory T cells (Tregs) and IL-10-producing CD4+ T cells. miR microarray analysis and polymerase chain reaction (PCR) validation from CD4+ T cells show treatment with resveratrol decreases the expression of several miRs (miR-31, Let7a, miR-132) that targets cytokines and transcription factors involved in anti-inflammatory T cell responses (Foxp3 and TGF-β). Transfection studies with miR-31 confirm that this miR directly regulates the expression of Foxp3. Lastly, analysis of public data from human patients with ulcerative colitis reveals that miR-31 expression is significantly increased when compared to controls. Togeth...Continue Reading

References

Feb 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·O AnnackerA Bandeira
Oct 19, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shannon Reagan-ShawNihal Ahmad
May 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Stephan SauerMatthias Merkenschlager
Jan 14, 2009·World Journal of Gastroenterology : WJG·Theresa A Mikhailov, Sylvia E Furner
Mar 25, 2010·Cancer Prevention Research·Xiangli CuiLorne J Hofseth
Jul 14, 2010·Journal of Gastroenterology and Hepatology·Tomohisa TakagiToshikazu Yoshikawa
Oct 31, 2012·Digestive Diseases·Sebastian FoerschMarkus F Neurath
Jun 5, 2013·Nutrition Reviews·Philip B BusbeePrakash S Nagarkatti
Jul 12, 2013·International Reviews of Immunology·Udai P SinghPrakash S Nagarkatti
May 13, 2014·The Journal of Pharmacology and Experimental Therapeutics·Ibrahim AltamemiMitzi Nagarkatti
Aug 26, 2014·Methods in Molecular Biology·George X Song-Zhao, Kevin J Maloy
Jun 16, 2015·World Journal of Gastroenterology : WJG·Jun YaoLi-Sheng Wang
Jun 10, 2017·Pharmacology Research & Perspectives·Ariane R GuthrieJessica A Martinez
Jun 11, 2017·Biochemical and Biophysical Research Communications·Zhaoyuan LiuHonglin Wang
Jun 11, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Joel PekowMarc Bissonnette
Dec 1, 2017·Journal of Autoimmunity·Patrick MaschmeyerMir-Farzin Mashreghi
Dec 2, 2017·Nutrition Research Reviews·Sandra NunesPaula Silva
Mar 8, 2018·Journal of Cellular and Molecular Medicine·Hasan AlghetaaPrakash Nagarkatti
Apr 19, 2018·Inflammatory Bowel Diseases·Katharina SchönauenAlexander Link
Jun 15, 2018·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Kathryn MirandaMitzi Nagarkatti
Jun 19, 2018·Journal of Crohn's & Colitis·Minhao YuMing Zhong
Jul 12, 2018·World Journal of Gastroenterology : WJG·Josef SýkoraJan Schwarz
Sep 12, 2018·Inflammatory Bowel Diseases·Carlos D MinacapelliKiron M Das
Sep 18, 2018·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Ian MorillaXavier Treton
Sep 19, 2018·Proceedings of the National Academy of Sciences of the United States of America·Loris R LopetusoTheresa T Pizarro
Nov 6, 2018·Frontiers in Immunology·Ibrahim Al BakirTrevor A Graham
Jan 2, 2019·World Journal of Gastroenterology : WJG·Elisabetta AntonelliGabrio Bassotti

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Citations

Nov 21, 2020·Journal of Immunotoxicology·Madelyn C HuangDori Germolec
Jan 24, 2021·Cardiovascular Research·Luke B RobertsGraham M Lord
Mar 1, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Yaxing ZhaoRuiyan Li
Jul 31, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yaoyao ChangHaitao Xiao
Aug 25, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Mehdi ZobeiriMohammad Abdollahi

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