Resveratrol Enhances Cardiomyocyte Differentiation of Human Induced Pluripotent Stem Cells through Inhibiting Canonical WNT Signal Pathway and Enhancing Serum Response Factor-miR-1 Axis

Stem Cells International
Hui LiuYongchun Zhang

Abstract

Resveratrol (trans-3,5,4'-trihydroxystilbene) (RSV) is a natural polyphenol with protective effects over cardiac tissues and can affect cell survival and differentiation in cardiac stem cells transplantation. However, whether this agent can affect cardiomyocytes (CMs) differentiation of induced pluripotent stem cells (iPSCs) is not yet clear. This study explored whether RSV can affect CMs differentiation of human iPSCs. Under embryoid bodies (EBs) condition, the effect of RSV on the change of pluripotent markers, endoderm markers, mesoderm markers, and ectoderm markers was measured using qRT-PCR. Under CM differentiation culture, the effect of RSV on CM specific markers was also measured. The regulative role of RSV over canonical Wnt signal pathway and serum response factor- (SRF-) miR-1 axis and the functions of these two axes were further studied. Results showed that RSV had no effect on the self-renewal of human iPSCs but could promote mesoderm differentiation. Under CM differentiation culture, RSV could promote CM differentiation of human iPSCs through suppressing canonical Wnt signal pathway and enhancing SRF-miR-1 axis.

References

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Citations

May 27, 2016·Cardiovascular Therapeutics·Hong DingQiuting Feng
Feb 24, 2017·Journal of Cellular Biochemistry·Na LiJinlian Hua
Aug 8, 2017·Cell Biology International·Ya-Wei GengWei-Ping Hu
Jul 29, 2018·Stem Cell Research & Therapy·Haissam Abou-SalehAli H Eid
May 22, 2019·International Journal of Molecular Sciences·Krista Minéia WartchowPatrícia Sesterheim
Apr 2, 2021·The Journal of Nutritional Biochemistry·Ibrahim JantanZeti-Azura Mohamed-Hussein

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Methods Mentioned

BETA
Transfection
Assay
Flow cytometry

Software Mentioned

ImageQuant

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