Abstract
Dysfunction of endothelial progenitor cells (EPCs) contributes to cardiovascular complications in diabetes, and resveratrol has been shown to improve EPC functions. Syndecan-4 (Synd4), a cell surface heparin sulfate proteoglycan, has been shown to promote neovascularization. Thus, the present study was performed to determine whether resveratrol promoted angiogenesis of EPCs by regulating Synd4. Late EPCs were isolated from human peripheral blood and stimulated with AGEs. Western blot showed that AGEs induced Synd4 shedding in a dose- and time-dependent manner. AGE-induced Synd4 shedding was partly reversed by NAC or resveratrol, along with normalized ROS production. Overexpression of Synd4 or pretreatment of resveratrol reversed AGE-impaired tube formation of EPCs and regulated the Akt/eNOS pathway. Furthermore, resveratrol suppressed Synd4 shedding via the inhibition of oxidative stress and improved tube formation of late EPCs via the regulation of the Synd4/Akt/eNOS pathway.
References
Dec 1, 2001·Cellular Signalling·M Simons, A Horowitz
Sep 21, 2007·Journal of Cellular Physiology·Sara Santana NunesVerônica Morandi
Sep 21, 2007·Clinical and Experimental Pharmacology & Physiology·Xiao-Bin WangKe-Jiang Cao
Oct 15, 2008·Molecular Cell·Chohreh PartovianMichael Simons
Aug 3, 2010·Cardiovascular Drugs and Therapy·Fatma AkarH Selcuk Surucu
Nov 25, 2011·Current Vascular Pharmacology·Nicolle KränkelUlf Landmesser
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Eugene JangAaron B Baker
Feb 22, 2012·Circulation Research·Gian Paolo FadiniStefanie Dimmeler
May 9, 2012·Journal of Molecular and Cellular Cardiology·Jun XieBiao Xu
Sep 15, 2012·Cellular Signalling·Rong Ju, Michael Simons
Aug 21, 2013·Journal of the American College of Cardiology·Dimitris TousoulisChristodoulos Stefanadis
Feb 4, 2014·Diabetes·Fang LiuAlex F Chen
Aug 15, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Raina RamnathSimon C Satchell
Jan 13, 2015·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Tram Thu VuongSvein O Kolset
Jun 13, 2015·Oxidative Medicine and Cellular Longevity·Kenneth Maiese
Jun 30, 2015·Diabetes Research and Clinical Practice· Idf Diabetes Atlas Group
Nov 1, 2015·Biochemical and Biophysical Research Communications·Pankaj K BagulSanjay K Banerjee
Jan 23, 2016·Oxidative Medicine and Cellular Longevity·Han WuBiao Xu
Mar 29, 2016·Biochemical and Biophysical Research Communications·Ran LiBiao Xu
Oct 25, 2016·Stem Cells·Jun XieBiao Xu
Nov 15, 2016·Molecular Medicine Reports·Xiaohua ShenLiang Xia
Jan 4, 2017·Journal of Diabetes·Yi Shi, Paul M Vanhoutte
Feb 1, 2017·Proceedings of the National Academy of Sciences of the United States of America·Santosh KumarKaikobad Irani
Aug 12, 2017·Journal of Cellular and Molecular Medicine·Xiaozhen DaiYi Tan
Citations
Apr 2, 2019·The FEBS Journal·Frank Gondelaud, Sylvie Ricard-Blum
Aug 29, 2019·Cartilage·Christelle SanchezYves Henrotin
May 10, 2019·Frontiers in Pharmacology·Xiaozhong HuangZhongxin Zhu
Apr 15, 2020·Biogerontology·Hai-Na ZhangJing Liang
Jul 3, 2021·International Journal of Molecular Sciences·Anne-Christine PeyterCatherine Yzydorczyk
Jan 9, 2022·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Gui WanZhenbing Chen